Blood products cell line selection

Recruit volunteers who met entrance requirements 2. Screen botanical ingredients for inhibition of IL-1 p production 3. Pilot clinical evaluation of IL-1 inhibitory lead candidate botanicals from activity 2 Establishing a clinical genotyped database Human monocyte cell lines stimulated in vitro with LPS Clinical + laboratory assay of peripheral blood mononuclear cells (PBMCs), and plasma Adequate number of healthy subjects with CRP = 2-10 mg/L and stratified by IL1 composite genotype Select lead candidate botanicals based on IL-1 protein inhibitory dose compared to untreated cultures IL1 gene expression in PBMCs and ex vivo IL-1 production in plasma from test subjects after 2-week dosing of candidate botanicals... 

The action of DMXAA in mice, like that of FAA, appears to be mediated by the induction of synthesis of cytokines, in particular TNF (70). A significant step toward the selection of DMXAA as a clinical candidate occurred when it was shown that DMXAA could upregulate TNF mRNA in human HL-60 cell lines while FAA was inactive (73). In addition, DMXAA, but not FAA, has been found to stimulate human peripheral blood leukocytes to produce TNF in culture. The responsiveness of leucocytes has been found to vary among individuals (74). These studies demonstrate that DMXAA, but not FAA, can stimulate TNF production in blood leukocytes from the majority of the population. 

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