Blood-brain barrier in vitro model

Blood-Brain Barrier In Vitro Models and Their Applications in Toxicology... 

Key words Blood-brain barrier, In vitro models, Cell culture, Endothelial cells, Glial cells, Permeability... 

Prieto P, Blaauboer BJ, de Boer AG, Boveri M, Cecchelli R, Clemedson C, Coecke S, Forsby A, Galla HJ, Garberg P, Greenwood J, Price A, Tahti H (2004) Blood-brain barrier in vitro models and their application in toxicology. The report and recommendations of ECVAM workshop 49. Altern Lab Anim 32(l) 37-50... 

Franke H, Galla H, Beuckmann CT (2000) Primary cultures of brain microvessel endothelial cells a valid and flexible model to study drug transport through the blood-brain barrier in vitro. Brain Res Brain Res Protoc 5 248-256... 

The relative ease with which such cocultures can be produced in large quantities facilitates the screening of new CNS drugs. This model provides an easier, reproducible and mass-production method to study the blood-brain barrier in vitro. 

Stanness, K.A.. Guatteo, E.. and Janigro, D. (1996) A dynamic model of the blood-brain barrier in vitro". Neurotoxicology, 17. 481-496. 

Santaguida S, Janigro D, Hossain M, Oby E, Rapp E, CucuUo L, 2006. Side by side comparison between dynamic versus static models of blood-brain barrier in vitro A permeability study. Brain Res. 1109 1. 

In vitro models mimicking the characteristics of the blood-brain barrier in a realistic manner provide a broad field of application in experimental, pharmaceutical, and clinical studies. In contrast to in vivo studies, which are mainly... 

The use of in vitro cell culture models for mechanistic studies and as permeability screens for the blood-brain barrier in the pharmaceutical Industry-Background and current status in the drug discovery process. Vascular Pharmacology, 38, 355-364. 

Lundquist S, Renftel M, Brillault J, Fenart L, Cecchelli R, Dehouck MP (2002) Prediction of drug transport through the blood-brain barrier in vivo a comparison between two in vitro cell models. Pharm Res 19(7) 976-981... 

Whereas the relationship of solute permeability with lipophilicity has been studied in a large number of in vivo systems (including intestinal absorption models [54,55], blood-brain [56 58] and blood nerve [59] barrier models, and cell culture models [60 62], to name just a few), numerous in vitro model systems have been developed to overcome the complexity of working with biological membranes [63-66]. Apart from oil-water systems that are discussed here, the distribution of a solute between a water phase and liposomes is... 

Tables 6.8-6.11 illustrate the wide range of C3 side-chain modified A -THC analogues that have been reported in the literature, together with associated in vitro and in vivo data. The affinity of classical cannabinoid analogues for the CBi receptor has been shown to correlate with depression of spontaneous activity and the production of antinociception, hypothermia and catalepsy in mice, and with psychomimetic activity in humans [93]. However, in some cases, there were unexplained differences between the observed trends in binding affinity and the trends in activity in mouse behavioural models. This may point to differences in efficacy among full agonists, partial agonists and antagonists/inverse agonists, or may reflect differences in in vivo metabolism or blood-brain barrier penetration or a combination of these factors.

Kramer, S. D. Hurley, J. A. Abbott, N. J. Begley, D. J., Lipids in blood-brain barrier models in vitro I TLC and HPLC for the analysis of hpid classes and long polyunsaturated... 

One should realize that the intracellular compartment as depicted in Figure 2 represents multiple cell types, whereas in vitro studies normally utilize a single cell type pertinent to characterizing specific attributes of drug transport in that cell system. The method of Shah et al. [51] would be of great benefit to investigating blood-brain barrier transport, consistent with a vascular-extravascular subcompartment brain model. 

Balazs, Z, Panzenboeck, U, Hammer, A, Sovic, A, Quehenberger, O, Malle, E, and Sattler, W, 2004. Uptake and transport of high-density lipoprotein (HDL) and HDL-associated alpha-tocopherol by an in vitro blood-brain barrier model. J Neurochem 89, 939-950. 

T. Terasaki, S. Ohtsuki, S. Hori, H. Takanaga, E. Nakashima, and K. Hosoya. New approaches to in vitro models of the blood-brain barrier drug transport. Drug Discov. Today 8 944-954 (2003). 

Chapter 17 In Vitro Models to Study Blood-Brain Barrier Function 399... 

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