Blocking agents gelatin

The proper choice of an application buffer can help to minimize any nonspecific binding due to undesired sample components. For example, coulombic interactions between solutes and the support can often be decreased by altering the ionic strength and pH of the application buffer. In addition, surfactants and blocking agents (e.g., Triton X-100, Tween-20, bovine serum albumin, and gelatin) may be added to the buffer to prevent nonspecific retention of solutes on the support or affinity ligand. 

Blocking agent Ovalbumin/gelatin Skimmed milk Tween-20... 

The choice of blocking buffer is sometimes critical for sensitive detection. Milk based blocking solutions are not recommended for use avidin-biotin system because milk contains biotin, which may directly cause competition with biotinylated antibody." Bovine Serum Albumin was not selected in this system in order to avoid the cross-reactivity. Therefore, gelatin was chosen as the blocking agent. 

Predominant protein blockers include bovine serum albumin (BSA), nonfat dry milk (NFDM), casein, and fish gelatin. NFDM, used at 0.1 0.5%, is inexpensive but preparations vary in quality. Some NFDM preparations contain histones that interfere with anti-DNA determinations or inhibitors of the biotin (strept)avidin interaction such as biotin itself. Casein is a chief component of NFDM and is often used alone as a blocking agent. 

On blots blocked with milk powder (5%), the ECL reaction apparently runs only weakly. Blots blocked with gelatin, on the other hand, exhibit a good ECL reaction, but also a higher background. The best blocking agents for ECL blots are said to be serums. Important acid inhibits peroxidases. 

However, in these cases the embolization agent has usually been resorbable gelatine sponge which does not produce as distal a block as non-resorbable particles and therefore may affect the uterus differently [13,115]. Encouragingly, ultrasound and MRl observation of the uterus following embolization demonstrates rapid revascularization of the normal myometrium and an essentially normal appearance of the endometrium on 3- to 6-month MRI exami-... 

---