Bioprocess dissolved oxygen concentration

Meilhoc E, Wittrup KD Bailey JE (1990) Influence of dissolved oxygen concentration on growth, mitochondrial function and antibody production of hybridoma cells in batch culture. Bioprocess Engineering 5 263-274. 

Bioprocess Control An industrial fermenter is a fairly sophisticated device with control of temperature, aeration rate, and perhaps pH, concentration of dissolved oxygen, or some nutrient concentration. There has been a strong trend to automated data collection and analysis. Analog control is stiU very common, but when a computer is available for on-line data collec tion, it makes sense to use it for control as well. More elaborate measurements are performed with research bioreactors, but each new electrode or assay adds more work, additional costs, and potential headaches. Most of the functional relationships in biotechnology are nonlinear, but this may not hinder control when bioprocess operate over a narrow range of conditions. Furthermore, process control is far advanced beyond the days when the main tools for designing control systems were intended for linear systems. 

Mixing time is a vital parameter affecting the efficiency of a bioreactor. The local concentrations of nutrients, toxins, dissolved oxygen, etc. are all dictated by the mixing process. A low mixing time ensures quick response to any additions (pH equalization, uniform distfibution of nutrients, etc.). This results in uniformity of concentrations of aU the important ingredients participating in the bioprocess. 

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