Biomedical Applications of RPLC

Karle et al. (K9) determined serum and plasma uridine levels in mice, rats, and humans by reversed-phase high-performance liquid chromatography. They report no difference in human plasma or serum uridine levels however, there is some individual variation in human serum uridine levels throu out the day but no consistent pattern. From this study they concluded that uridine levels appear to be regulated and are not a direct reflection of dietary intake of uridine. 

Realization of the biological significance to tRNAs has stimulated research directed at the elucidation of the many aspects of these 

Gehrke s group (G4) analyzed the urine of breast cancer and leukemia patients by RPLC. They report elevated levels of N -acetylcytidine in the breast cancer samples. They also report similar findings of elevated levels of N, N -dimethylguanosine, 1-methylguanosine, 1-methylinosine, and pseudouridine in the urine of patients with Burkitt s lymphoma, lung, colon, breast, and other types of cancer (Wl, W2). 

Moreover, in studies of patients with chronic lymphocytic leukemia and lung cancer, Hartwick et al. (H7) reported distinctive changes in their serum RPLC profiles vis-a-vis normal profiles. 

Reversed-phase liquid chromatography has been used by Veening and co-workers (K22, S18) to monitor dialysate, serum, and urine from patients on artificial kidney machines. They report that RPLC is a reliable method for monitoring blood composition during dialysis. 

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