Bioluminescent report strains

Layton, A.C., Muccini, M., Ghosh, M.M., and Sayler, G.S., Construction of a bioluminescent reporter strain to detect polychlorinated biphenyls, Appl. Environ. Microbiol., 64, 5023-5026, 1998. 

Sagi E., Hever N., Rosen R., Bartolome A.J., Premkumar J.R., Ulber R., Lev O., Scheper T., Belkin S., Fluorescence and bioluminescence reporter functions in genetically modified bacterial sensor strains, Sens. Actuat B 2003 90 2-8. 

If bacteria producing viscosinamide were added to colonies of the reporter strain the bioluminescence of the fungus increased greatly compared to controls (Fig 5.7) indicating that the effect of viscosinamide is to make holes in the fungal membrane. 

Ralstonia eutropha strain a whole-cell biosensor developed for the detection of bioavailable concentrations of Ni2+ and Co2+ in soil samples, the regulatory genes are fused to the bioluminescent lux CDABE reporter system. This modified bacteria used to study the accumulation of above said metals [17],... 

A bioluminescent whole-cell reporter Ralstonia eutropha strain developed by using with a tfdRP(DII)-luxCDABE fusion gene for detection of weedicides like 2,4-dichlorophenoxyacetic acid and 2,4-dichlorophenol in the contaminated soils [19]. 

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