Bioluminescence materials

The bioluminescence of the intermediate, measured in the presence of long-chain aldehyde, corresponds to an activity of 5.7 x 1013 quanta sec-1 mg-1, which is close to the activity of the starting material (4.5 x 1013 quanta sec-1 mg-1). This difference in activity shows that the isolation of the intermediate also leads to purification of the enzyme. 

Curtis, C., Lima, A., Lozano, S.J. and Veith, G.D. (1982) Evaluation of a bacterial bioluminescence bioassay as a method for predicting acute toxicity of organic chemicals to fish. In Aquatic Toxicology and Hazard Assessment Fifth Conference, ASTM STP 766, Pearson, J.G., Foster, R.B. and Bishop, W.E. (eds), pp. 170-178. American Society for Testing and Materials, Philadelphia, PA. 

In comparison to equivalent optical detection methods using whole cell biosensors for water toxicity detection, these results proved to be more sensitive and produce faster response time. Concentrations as low as 1% of ethanol and 1.6 ppm of phenol could be detected in less than 10 min of exposure to the toxic chemical, whilst a recent study [11] which utilized bioluminescent E.coli sensor cells, detected 0.4 M (2.35%) ethanol after 220 min. An additional study [1] based on fluorescent reporter system (GFP), enabled detection of 6% ethanol and 295 ppm phenol after more than one hour. Cha et al [12] used optical detection methods of fluorescent GFP proteins, detected 1 g of phenol per liter (1,000 ppm) and 2% ethanol after 6 hours. Other studies [13] could not be directly compared due to different material used however their time scale for chemicals identification is hours. 

Figure 1. Bugdeath test rig modified to enable real-time quantitation and positional detection of bacterial bioluminescence during heat treatment. The sample holder is shown in the lowered position and light exclusion material is not in place.

The information system contains several modules the Web-portal Bioluminescence and luminous organisms , the database BIOLUMBASE of natural and genetically modified luminous microorganisms, the electronic catalogue of cultures maintained in CCIBSO, information concerning history of bioluminescence studies, different properties and expressions of bioluminescence, methods and devices to measure bioluminescence, reagents for bioluminescent analysis and also applied programs for administration of the information system (Fig.l). The developed structure of the database will reflect all types of properties and communications of relevant material,... 

We were fortunate to have oral and poster presentations given by scientists from 19 countries, as well as active participation from industrial exhibitors. The sessions included luciferase-based bioluminescence, photoprotein-based bioluminescence, fundamental aspects and applications of chemiluminescence, luminescence imaging, fluorescence quantum dots and other inorganic fluorescent materials, phosphorescence and ultraweak luminescence, instrumentation and new methods. 

These exceedingly unstable substances, which are invoked as the active intermediates in bioluminescence,20 were first prepared and isolated by Adam and Liu.2 The /-butyl system (2a) was the originally synthesized derivative by dehydrative cyclization of the a-hydroperoxy acid (6) by dicyclohexylcarbodiimide (DCC) [Eq. (5)]. It was not possible to isolate the pure material in view of its great thermal instability but its characteristic carbonyl band at 1875 cm-1 in the infrared (IR) served as unequivocal structure identification of these novel cyclic peroxides. 

Research on QDs has evolved from eleetronie materials scienee to biological applications. A broad variety of synthetic methods has been developed for the preparation of QDs, conjugation with biomolecules, and apphcation as bioluminescent... 

Dual-Color Bioluminescence Live-Cell Imaging 2.2.7 Required Materials... 

Thomulka, K. W., McGee, D. J., Lange, J. H. 1993. Detection of biohazzardous materials in water by measuring bioluminescence with the marine organism Vibrio harveyi. Journal Environmental Science and Health. A28 2153-2166. 

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