Biocompatibility cell growth inhibition

A second index of biocompatibility was the quantitative analysis of cell growth inhibition, again on mouse fibroblast L929 cells, induced by the liquid components of the adhesive system and water extracts of two solid crosslinked materials. Table IV is a summary of the percent inhibition of cell growth (percent ICG). The mean protein values at 4 °C have been subtracted from the mean protein values (five test samples) at 37 °C for each treatment condition. The percent inhibition of cell growth (percent ICG) is shown for each treatment condition. The precision of the assay is approximately 10%. 

Biocompatibility at the Cell Culture Level. The liquid components, MAP and catechol oxidase, and extracts of two solid crosslinked materials were tested for cell culture toxicity and growth inhibition. The evaluation of cell culture cytotoxicity in an agar overlay method detects the impact on cells of any freely... 

In another study, an amphiphilic hyperbranched multiarm copolymer [H40-star-(PLA-b-PEP-OH)J was synthesized through a two-step ROP procedirre (Figure 12). First, Boltom H40 was used as the macroinitiator for the ROP of l-1A to form the intermediate (H40-star-PLA-OH). Then, the ROP of EEP was further initiated to produce H40-star-(PLA-b-PEP-OH). Benefiting from the amphiphilic stmcture, H40-star-(PLA-b-PEP-OH) was able to self-assemble into micelles in water with an average diameter of 130 nm. In vitro evaluation of these micelles demonstrated their excellent biocompatibility and eftident cellular uptake. DOX-loaded micelles were investigated for the proliferation inhibition of HeLa human cervical cardnoma cell line, and the DOX dose requited for 50% cellular growth inhibition was found to be 1 pg ml". ... 

Because immature dendritic cells display abundant mannose receptors for endo-cytosis or phagocytosis, mannose can be applied to active targeting of chitosan nanoparticles. For interleukin-12 (lL-12) gene delivery to dendritic cells around the tumor cells, mannose ligand was considered for modification of the chitosan nanoparticles [35]. The study showed that mannosylated chitosan and IL-12 plasmid complexes could inhibit tumor growth, and that biocompatible mannosylated chitosan nanoparticles were effective as a gene carrier for cancer immunotherapy. 

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