Bioassay-directed fractionation techniques

Sezik E, Aslan M, Yesilada E, Ito S. Hypoglycaemic activity of Gentiana olivieri and isolation of the active constituent through bioassay-directed fractionation techniques. Life Sci 2005 76(ll) 1223-38. 

For example, Legzdins and co-workers (1994) used the bioassay-directed fractionation and chemical analysis technique to isolate, identify, and quantify 2-nitrofluoranthene in extracts of ambient particles collected in Hamilton, Ontario, Canada. They found it accounted for 70% of the total nonpolar direct bacterial mutagenicity (strain YG1021, standard reversion assay, Maron and Ames, 1983). 

During the past three years several procedures have been developed for simplifying the analysis of complex environmental samples. These techniques have included "Bioassay Directed Chemical Analysis," (15) which uses short-term bioassays for determining which chemical fractions should be prioritized for detailed chemical analysis and "Survey Chemical Analysis", which utilizes analytical techniques such as hl h resolution mass... 

In order to identify compounds responsible for specific effects (i.e., endocrine disrupting or AhR hgands) observed in field studies, TIE or bioassay directed analysis approaches have increasingly been apphed over the last decade. In such approaches, sensitive bioassays are used to direct the fractionation of a sample extract until its complexity is sufficiently reduced to enable identification of those compoimds responsible for the activity measured in the bioassay. This strategy is based on differential extraction and fractionation methods and identification by chemical and biochemical analysis. TIE is a well-established technique having been originally developed by... 

M. G. Weller, A unifying review of bioassay-guided fractionation, effect-directed analysis and related techniques. Sensors 12 9181-9209, 2012. 

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