Bifunctional chelating

Fig. IS. DTS derivatives as used in the bifunctional chelating approach to label proteins with technetium...

Fig. 8. Example of "mTcO-DADT bifunctional chelating agents R = sidechain for attachment to biomolecule...

E. Benoist, A. Loussouam, P. Remaud, J. C. Chatal, and J. F. Gestin, Convenient and simplified approaches to V-monoprotected triaminopropane derivatives. Key intermediates for bifunctional chelating agent synthesis, Synthesis (1998) 1113-1118. 

Add a quantity of the SCN-Bzl-DTPA bifunctional chelating agent to obtain the desired molar excess of label over the amount of dendrimer present. The optimal ratio may be determined experimentally by preparing a series of dendrimer-chelate conjugates using different molar ratios and choosing the one that works the best in the intended application. 

Other chelate-dendrimer constructs may be prepared using alternative bifunctional chelating agents according to Chapter 10. 

Determine the amount of adsorbed protein on the particles by using a suitable protein assay technique, such as the bifunctional chelating agents (BCA) Protein Assay (Thermo Fisher). 

Figure 20.18 The bifunctional chelating reagent DTPA may be used to modify amine groups on antibody molecules, forming amide bond linkages. Indium-111 then may be complexed to the chelator group to create a radiolabeled-targeting reagent.

Figure 28.17 FeBABE is a bifunctional chelating agent containing an EDTA group on one side and a thiol-reactive bromoacetyl group on the other end. The EDTA group is coordinated with an iron ion.

Brechbiel, M.W., McMurry, T.J., and Gansow, O.A. (1993) A direct synthesis of a bifunctional chelating agent for radiolabeling proteins. Tetrahedron Lett. 34, 3691-3694. 

Moi, M.K., Meares, C.F., McCall, M.J., Cole, W.C., and DeNardo, S.J. (1985) Copper chelates as probes of biological systems stable copper complexes with a macrocyclic bifunctional chelating agent. Anal. Biochem. 148, 249-253. 

Renn, O., and Meares, C.F. (1992) Large scale synthesis of the bifunctional chelating agent 2-p-nitroben-zyl-l,4,7,10-tetraazacyclododecane-N,N, N"N" -tetraacetic acid and the determination of its enantiomeric purity by chiral chromatography. Bioconjugate Chem. 3, 563-569. 

Subramanian, R., and Meares, C.F. (1991) Bifunctional chelating agents for radiometal-labeled monoclonal antibodies. In Cancer Imaging with Radiolabeled Antibodies (D.M. Coldenberg, ed.), pp. 183-199. Kluwer, Boston, MA. 

In the schematic representation shown in Fig. 4, the radionuclide core is supported by a bifunctional chelating ligand or encapsulating unit, which may be intrinsically fluorescent and decorated with one (or more) biologically active molecules that can bind to specific receptor sites in vivo. Such examples... 

Some of the copper-labeled bifunctional chelators reported to date have shown an enhanced stability in a biological environment and decreased transchelation (Fig. 14) with respect... 

The purpose of a bifunctional chelator is to link a radiometal covalently to a targeting molecule such as a monoclonal antibody or a receptor-binding peptide. In the case of rhenium, the molecule therefore... 

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