Bacterial cellulose purification

This study showed that the bacterial cellulose derived fix)m coconut and pineapple juices can be converted efficiently to bacterial cellulose by the supplementation of yeast extract and ethanol under static fermentation conditions at 30 °C. Bacterial celluloses produced from all strains are growth associated products. Coconut juice seems to be a better substrate than pineapple juice. In view of energy consumption, the productivity of BC on this medium is high, which makes the production costs lower than expected. It is also clear that different A. xylinum strains produce different BC content levels under the same inoculation volumes and under static cultivation conditions. These results suggest that bacterial cellulose pellicles of all strains appear to be easily applied to use in many applications such as food, paper, and textile industries, without requiring additional steps of decolorization and purification. Furthermore, the properties of cellulose, in tenns of crystallinity, high water-absorption capacity, and mechanical strength of the reported strains, have additional applications in cosmetics and medicine. 

Gea, S., Reynolds, C.T., Roohpour, N., Wiijosentono, B., Soykeabkaew, N., Bilotti, E., et ah, 2011. Investigation into the structural, morphological, mechanical and thermal behavior of bacterial cellulose after a two-step purification process. Bioresour. Technol 102, 9105-9110. 

Bacterial cellulose is a special kind of cellulose which is produced by microorganisms. It has unique properties. The biosynthesis and purification of bacterial cellulose have been reviewed (19). 

The more suitable purification method for the bacterial cellulose calls upon distilled water, sodium hydroxide and sodium hypochlorite solutions, since it guarantees the elimination of bacteria cells and culture medium residues from the membrane. For clinical applications, the suitable sterilization processes are gamma radiation and ethylene oxide treatment, but the latter should be avoided for bacterial cellulose dry or wet membrane, because it can cause allergic reactions, mostly when used internally. 

Pandey L.K., Saxena C., Dubey V, Studies on pervaporative characteristics of bacterial cellulose membrane. Separation and Purification Technology, 42, 2005, 213. 

Bacterial cellulose-modified sisal fibre with purification 12.0 0.9 310 32 4.1 0.5... 

Bacterial cellulose-modified hemp fibre with purification 8.0 0.6 130 12 2.9 0.2... 

Purification indicates the extraction of post-bacterial cellulose-modified sisal fibres with NaOH at 80° C... 

Colvin, R., Leppard, G., 1971. Purification of the precursor of bacterial cellulose. Journal of Polymer Science Part C Polymer Symposia 36 (1), 417—424. 

Recently, bacterial cellulose has gained much attention. It is produced by a specific genera of bacteria and does not require any extra purification. What is more, it exhibits some exclusive properties that are not offered by plant cellulose [23]. This type of cellulose will be further discussed in the next subsection. 

The construction of hybrid proteins containing bacterial CBDs may provide a cheap generic method for enzyme immobilization and/or purification using cellulosic matrices. The CBD can be fused at the amino or carboxyl terminus, as in the parent cellulase, to suit individual applications. We have constructed model fusion proteins using the C. fimi CBDs to demonstrate this potential. 

Nilesh A., Kamat, M., Arvind, L. (2004). Expanded bed affinity purification of bacterial a-amylase and Cellulase on composite substrate analogue-Cellulose matrices. Process Biochemistry, 39, 565-570. 

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