Azinphos ethyl

Dichlorovos, phorate, dimethoate, diazinon, disulfoton, methyl-parathion, malathion, parathion, azinphos-methyl, azinphos-ethyl, and so on... 

Fig. I Reflectance scan of a chromatogram track with 300 ng substance per chromatogram zone 1 = azinphos methyl, 2 = azinphos ethyl, 3 = parathion methyl, 4 = fenitrothion, 5 = parathion ethyl + phoxim.

Azinphos ethyl (h/ f 20-25), malathion (h/ f 40-45) and diazinone (h/ f 47-52) yielded white chromatogram zones on a blue background immediately. Before in situ quantitation the chromatogram was dried in the air until no film of moisture could be seen on the layer surface. It was then dried completely in a stream of warm air whereby the blue coloration of the background changed to brown (Fig. 1). The visual detection limits were 200 ng substance per chromatogram zone. 

Fig. 1 chromatogram of the thiophosphate insecticides (each ca. SOO ng) after treatment with dipping solutions I and II (A) before and (B) after complete drying of the TLC plate. TVacks 1 and S mixture Track 2 azinphos ethyl Track 3 malathion Track 4 diazinone. 

Fig. 2 Reflectance scan of a chromatogram track with 500 ng azinphos ethyl (1), 580 ng malathion (2) and 590 ng diazinone (3) per chromatogram zone.

Fig. 1 Reflectance scan of a chromatogram track with 100 ng each substance per chromatogram, one 1 = demeton-S-methyl sulfone, 2 = dimethoate, 3 = demeton-S-methyl, 4 = triazophos, 5 = azinphos-methyl, 6 = azinphos-ethyl, 7 = malathion, 8 = parathion-methyl and 9 = para-...

Azinphos methyl (h/ f 15-20), azinphos ethyl (liRf 20-25, parathion methyl (hiif 40-45), fenitrothion (h/ f 45—50), parathion ethyl (hR 60-65) and phoxim QxRf-. 60-65) appear as red-colored chromatogram zones on a colorless background. 

New a.s. are active substances not on the market of EC in protection products before 25 July 1993. Noninclusion has been decided for the following as after evaluation azinphos-ethyl, chlozohnate, chlorfenapyr, cyhalothiin, dinoterb, DNOC, fentin-acetate, fentin-hydroxide, fenvalerate, ferbam, lindane, monolinuron, parathion, permethrin, propham, pyrazophos, quintozen, tecnazen, zineb. 

Fig.4 SPE-LC-DAD analysis of a wastewater sample. Peak identification number and peak retention times (min) (I) azinphosmethy, (11) parathion-methyl, (4) malathion, (3) feni-trothion, (8) azinphos-ethyl, (6) chlorphenvinphos, (10) parathion-ethyl, (7) diazinon [from ref. 21]...

Mevinphos I, dimethoate, mevinphos II, dichlorvos, diazinon, paraoxon, azinphos-methyl, fenamiphos, parathion-methyl, malathion, fenitrothion, azinphos ethyl, fenthion, parathion-ethyl, coumaphos, phorate, phoxim, disulfoton, chlorpyriphos, ome-thoate, monocrotophos, vamidothion, bromophos ethyl, metoxichlor, DDT... 

Azinphos methyl, phosmet, parathion methyl, azinphos ethyl, fenitrothion, parathion, diazinon... 

Animal tissues, Fenitrothion, azinphos ethyl, chlorfenson, tetradifon, dicofol Carbamate... 

Water (E) Mevinphos, (Z) mevinphos, dichlorvos, azinphos methyl, parathion methyl, mala-thion, fenitrothion, azinphos ethyl, parathion, fenthion, chlorfenvinphos, diazinon ... 

Fig. 4 Chromatograms of a blank apple sample (top) and the same apple sample spiked with 27 of the 31 organophosphorus at the following concentration levels (bottom). 1 = paraoxon methyl 500 2 = malaoxon 6400 3 = paraoxon ethyl 800 4 = metidathion 500 5 = azinphos-methyl 200 6 = phosmet 200 7 = parathion methyl 800 8 = malathion 2000 9 = triazophos 200 10 = fenitrothion 800 11= azinphos ethyl 200 12 = chlorfenvinphos 200 13 = quinalphos 200 14 = parathion-ethyl 400 16 = ethrimphos 1000 17 = diazinon 800 18 = coumaphos 800 19 = fonophos 200 21 = phoxim 400 23 = chlorpyriphos-methyl 500 24 = disulfoton 2000 26 = isofenphos 500 27 = fenchlorphos 500 28 = temephos 400 29 = chlorpyriphos ethyl 200 30 = pyrimiphos-ethyl 200 31 = carbophenothion-ethyl 400. The chromatography was carried out with the Altima column using CH3CN/H20 as mobile phase in gradient elution CHjCN from 28 to 39% in 20 min, and then from 39 to 88% in 40 min. (From Ref. 59.)...

Several qualitative and quantitative immunochemical methods and their application to the analysis of environmental samples have been described for OP insecticides, a family that includes widely used pesticides such as azinphos-ethyl/methyl, dichlorvos, fenitrothion or fenthion, malathion, mevinphos, and parathion. Mercader and Montoya202 produced monoclonal antibodies against azinphos-methyl and developed an ELISA that was used for the analysis of water samples from different sources, reaching detectability levels near 0.05 pg I. Watanabe et al.203 reported the production of polyclonal antibodies and ELISA procedures to analyze fenitrothion in river, tap, and mineral water (LOD = 0.3 pg L ). Banks et al.204 produced polyclonal antibodies against dichlorvos, an organophosphate insecticide used for stored grain, which also cross-reacts with fenitrothion. Nishi et al.205 reported the first immunoassay for malathion. Residues of this insecticide have... 

Azinphos-ethyl/ ELISA Well, tap, channel, cistern, 0.05 pg L-> 0.33 pg L-1 202... 

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