Autoradiograph

Physical methods Physical methods include photometric absorption and fluorescence and phosphorescence inhibition, which is wrongly referred to as fluorescence quenching [1], and the detection of radioactively labelled substances by means of autoradiographic techniques, scintillation procedures or other radiometric methods. These methods are nondestructive (Chapt. 2). 

Comar, C.L., Lotz, W.E. and Boyd, G.A. 1952 Autoradiographic studies of calcium, phosphorus, and strontium distribution in the bones of the growing pig. American Journal of Anatomy 90 113-125. 

Since these studies utilized autoradiographic techniques, it was important to determine the chemical nature of the material measured using radiochemical procedures. Using punch biopsies of the brain slices which were measured autoradiographically, it was shown using thin-layer chromatography that about 30% of the radioactivity was associated with unchanged BCNU (14). It was therefore concluded that the measurements described above accurately reflect brain concentrations of BCNU. 

R., Chasin, M., Tamargo, R., and Colvin, O, M., The intracerebral delivery of BCNU with surgically implanted biodegradable polymers A quantitative autoradiographic study, Proc. 

Fig. 3. Autoradiograph of SDS-PAGE of in vitro translated dihydrofolate reductase (DHFR) in the wheat germ cell-free protein synthesis systems with (n) 4 pi of ribosome fiaction, (III) 4 pi of 0 -40 % ammonium sulfate fraction, or (IV) 4 pi of 40 - 60% ammonium sulfate fraction, respectively. Lane I is control dihydrofolate reductase produced in the normal wheat germ cell-free protein synthesis system.

Pollard, H, Moreau, J, Arrang, JM and Schwartz, JC (1993) A detailed autoradiographic mapping of histamine H3 receptors in rat brain areas. Neuroscience 52 168-189. 

Effects of MDMA and MDA on Brain Serotonin Neurons Evidence from Neurochemicai and Autoradiographic Studies... 

Autoradiographic Studies on [ HJParoxetine-Labeled Serotonin Uptake Sites... 

In vitro autoradiographic studies of [ HJparoxetine-labeled serotonin uptake sites in control and MDMA-treated brains were earried out as previously deseribed (De Souza and Kuyatt 1987) to assess the neuroanatomie loealization of lesions induced by MDMA. Substantial reduetions in serotonin uptake sites (50 to 100 percent decreases) were observed in all areas of eerebral cortex as early as 18 hours after a 4-day treatment... 

De Souza, E.B., and Kuyatt, B.L. Autoradiographic localization of [ HJparoxetine-labeled serotonin uptake sites in rat. Synapse 1 488-496,... 

Kuhar, M.J., and Aghajanian, G.K. Selective accumulation of H-serotonin by nerve terminals of raphe neurons An autoradiographic study. 

Azmitia, E.C., and Segal, M. An autoradiographic analysis of the differential ascending projections of the dorsal and median raphe nuclei in the rat. J Comp Neurol 179 641-668, 1978. 

Conrad, L.C.A. Leonard, C.M. and ffaff, D.W. Connections of the median and dorsal raphe nuclei in the rat Autoradiographic and degeneration study. J Comp Neurol 156 179-206. 1974. 

Barber P.C. and Raisman G. (1974). An autoradiographic investigation of the projection of the vomeronasal organ to the accessory olfactory bulb in the mouse. Brain Res 81, 21-30. 

Lehman M.N. and Winans S.S. (1982). Vomeronasal and olfactory pathways to the amygdala controlling male hamster sexual behavior — autoradiographic and behavioral analyses. Brain Res 240, 27-41. 

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