Autoradiographic imaging

Exposure of hot particle contaminated soil (sampled 15 km north of the Chernobyl Nuclear Power Plant) to film X, Y and Z of a Differential Autoradiographic Imaging Sandwich. The original film size is 85 x 10 x 10 mm, exposure time was 14 days. Area (a) shows irradiation from a 90Sr dominated hot particle whereas irradiation in area (b) is caused by a 137Cs dominated hot particle. 

Figure 7.11. The effects of exposure temperature on protein synthetic patterns of isolated gill tissue from specimens of 13°C-acclimated Tegula funebralis. Autoradiographic images illustrate newly synthesized (35S-labeled) proteins of several size classes (molecular mass standards are shown in the left lane). Two specimens from each temperature of incubation are shown. At temperatures above 24°C, synthesis of heat-shock proteins in the molecular mass ranges of 38, 70, 77, and 90 kDa is induced. Hsp synthesis becomes an increasingly large fraction of protein synthesis as exposure temperature increases, and by 38°C, only synthesis of hsp70 is observed. By 39° C, no protein synthesis takes place. (Figure modified after Tomanek and Somero, 1999.)...

In an attempt to determine the localization of glycogen in the liver, could there be any problems of interpretation of the electron microscopic autoradiographic images if [3H]glucose were used as the radioactive precursor molecule of glycogen ... 

Belayev L, Zhao W, Busto R, Ginsbeig MD (1997) Tiansient middle cerebral artery occlusion by intraluminal suture I. Three-dimensiontil autoradiographic image-analysis of local cerebral glucose metabolism-blood flow interrelationships during ischemia and early recirculation. J Cereb Blood Flow Metab 17 1266-1280... 

CHEN, X., et al., MicroPET and autoradiographic imaging of GRP receptor expression with Cu-DOTA-[Lys ]Bombesin in human prostate adenocarcinoma xenografts, J. Nucl. Med. 45 (2004) 1390-1397. 

Figure 8 Representative autoradiographic images taken from cross sections of rabbit eyes injected intravitreously with radiolabeled INS37217 (3H-INS37217 at 3 mg per eye) showing the distribution of radiolabeled signal in various ocular structures at the postinjection time points indicated. Radioactivity from INS37217 or its metabolites is distributed throughout the entire vitreous within 15 minutes and is largely absent by 48 hours.

Fig. 5. Cell-free PrP conversion reaction. The right panel is a phosphor-autoradiographic image of an SDS-PAGE gel showing only the radiolabeled hamster PrP molecules in the reaction with and without PK digestion after incuhation in the presence or absence of unlabeled PrP-res. The PrP-res (PrP ) was isolated from scrapie-infected hamster brain tissue. Details of the reaction can be found in (Kocisko et at., 1994).

Fig. 6. In situ conversion reaction in brain slices on glass slides (Bessen et al., 1997). A distinct autoradiographic image is seen with scrapie-infected, but not uninfected control, brains. If the brain slices are solubilized after the conversion reaction and analyzed by SDS-PAGE/autoradiography, then PK-resistant S-PrP conversion products similar to those produced in cell-free conversion reactions (see Fig. 5) are observed. Higher magnification images (bottom panels) show that the pattern of in situ conversion product closely matches that of immunohistochemical staining for PrP-res in regions known to contain either amyloid plaques or diffuse apparently nonamyloid deposits.

The development of serotonergic innervation to the cerebral cortex has been characterized in neonatal rats by immunohistochemical techniques and autoradiographic imaging. These experiments demonstrate early serotonergic innervation that could be involved in a transient physiological role in sensory areas of the cortex or exert a trophic influence on the development of cortical circuitry and thalamocortical connections [76]. 

Figure 4.2 Estimation of diffusion coefficients by autoradiography of tissue sections, (a) Digitized autoradiographic image of radiolabeled NGF released from a polymeric implant in a rat brain, reproduced from [18]. (b) The resulting concentration profile, obtained by scanning intensities from the autoradiographic image, and using calibrated standards to determine the local concentration the solid line indicates Equation 3-58.

Ensure that the autoradiographic images are not overexposed (i.e., black, or near black) if they are to be assessed densitometrically. 

Figure 3.7. Enhanced production of transuranium nuclides at the fuel pellet rim (autoradiographic image)...

The radial distribution of the fission products over the cross section of the fuel pellet is primarily governed by the profile of the thermal neutron flux. However, there are two effects leading to variations in the distribution of some of the fission products. The first one of these effects is the preferential formation of plutonium in the outermost pellet zones due to epithermal neutron capture in the nucleus. This effect is more pronounced in high-bumup fuel than in fuel with a lower burnup. Fig. 3.7. shows an alpha autoradiographic image of a fuel pellet cross section where the enhanced a activity in the outer ring can be seen it must nonetheless be pointed out that the greatest fraction of this a activity is not due to the... 

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