Autoradiographic detection

Laskey RA, Mills AD. 1977. Enhanced autoradiographic detection of P and I using intensifying screens and hyper-sensitized film. FEBS Lett 82 314-316. 

Figure 3. Autoradiographic detection of naphthalene degradative bacterial colonies from MGP soil enrichments used as inoculum for continuous stirred soil slurry bioreactors.

Laskey, R. A. and Mills, A. D. (1977) Enhanced autoradiographic detection of 32P and l25I using intensifying screens and hypersensitized film. FEES Lett 82,314—316... 

Fig. 10. 5-HT6 receptors in the rat brain. (A-C) Autoradiographic detection of 5-HT6 mRNA as labeled by in situ hybridization using a 32P-labeled oligonucleotide probe. Autoradiographic dark silver grains denote the presence of hybridizing 5-HT6 mRNA. Abbreviations CA1 CA1 hippocampal field CA3 CA3 hippocampal field CPu caudate putamen DG dentate gyrus Tu olfactory tubercle. Scale bar 3 mm.

Figure 17 shows the autoradiographic detection of the 3P-labeled polydeoxyadenosine homopolymer sample, which is the same sample as shown in Figures 15 and 16. The gain in sensitivity is several orders of magnitude. Indeed, it is even possible to discern S3P-labeled material "trapped" in the gel matrix between peaks. Clearly this method is the method of choice when sensitivity is an issue. The same method can also be applied to free-solution capillary electrophoresis. 

In addition to the detection of antigens and antibodies, EIA will, undoubtedly, play an increasingly important role in molecular biology. For example, the bio-blot method (Leary et al., 1983) for the detection of DNA-DNA or DNA-RNA duplexes on nitrocellulose membranes offers important advantages over conventional procedures in which radioactive probes are used and autoradiographic detection. In this method, biotinylated DNA probes are prepared by nick translation (Rigby et al., 1977) in the presence of biotinylated analogs and hybridized with the DNA or RNA on filters. Biotin is then detected by avidin-labeled enzyme (Section 3.1). 

Figure 10.5 In vitro autoradiographic detection of Afl amyloid deposits with [ C]4 in TgCRNDS mouse brain sections. Clear differences between histochemically characterized TgAp-I- (left panel) and TgAp— (right panel) brains are readily observable [36]. (Reproduced with permission from Elsevier.)...

Berlin, M., and R. Rylander. 1963. Autoradiographic detection of radioactive bacteria introduced into sea water and sewage. J. Hyg. 61 307-315. 

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