Automated High-Throughput DNA Purification Systems

The degree of automations has literally revolutionized nucleic acid purification. The core of these systems are the automated liquid-handling workstations that involve the movement of multiple probes in Cartesian axes (x, y, z) over a deck configured with lab ware, such as microplates, tube racks, solvent reservoirs, washbowls, and disposable tips. They have the ability of aspirating and dispensing solvents from a source to a destination. Devices such as vacuum manifolds, heating blocks, and shakers have also been modified to handle 96-well (or more) plates. The multiprobe liquid handlers have a variable tip spacing that allows them to expand their tip-to- 

A multitip pipetter dispenses sample lysates into the multiwelled SPE/ 

SPRI plate. The multiwelled plates move through vacuum manifolds, shakers, heating blocks, and magnetic plate shelf. Gripper arms and conveyer belts are used as necessary. 

In-built automated vacuum system, or centrifugation system aids the washing step. 

Lift and transfer stations aid in storing the DNA samples into chillers. Fluorescent readers perform quantification, barcode readers identify the samples into the control software. 

---