Syntheses, chemical automated

The automated chemical synthesis of moderately long oligonucleotides (about 100 nucleotides) of precise sequence is now a routine laboratory procedure. Each synthetic cycle takes but a few minutes, so an entire molecule can be made by synthesizing relatively short segments that can then be ligated to one another. Oligonucleotides are now indispensable for DNA se-... 

Porco JA, Deegan TL, Devonport W, Gooding OW, Labadie JW, Mac Donald AA, Newcomb WS, Van Eikeren P, Automated chemical synthesis chemistry development on the Nautilus 2400 , Drugs of the Future, 23 71-78, 1998. 

Clark-Lewis, I., Aebersold, R. A., Ziltener, H., Schrader, J. W., Hood, L. E., and Kent, S. B. H. (1986) Automated chemical synthesis of a protein growth factor for hemopoietic cells, interleukin-3. Science 231,134-139. 

Once APMs have been created in the AIMS, a process control and monitoring software (PCMS) module can be constructed that provides for two-way communication between a database that contains the APMs and the instrument control software. This software allows for the retrieval of APM data from the AIMS by a production scientist and transfer to the instmment control software. Electronic transfer of APMs between unit operations in this manner allows for (a) charting of APM progress on the production floor and (b) collection of APM audit data. The PCMS will facilitate maximal process control for the automated chemical synthesis platform. Functionalities for the array information management and process control management software are summarized in Table 3. 

Our studies have focused on the development of automated chemical synthesis and screening systems, then on the preparation and analysis of diverse photoelectrochemical libraries of metal-oxides with semiconducting and other properties suitable for... 

This protocol describes automated chemical synthesis of oligodeoxynucleo-tide by means of the phosphoramidite method. The following procedure was developed for the ABI 394 DNA-RNA synthesizer at 0.2 mol. 

Schaller H, Weiman G, Lerch B, Khorana HG. The stepwise synthesis of specific deoxypolynucleotides. J Am Chem Soc 85, 3821-3827, 1963. Usman N, Ogilvie KK, Jiang M-Y, Cedergren RJ. Automated chemical synthesis of long oligoribonucleotides using 2 -0-silylated libonucleoside 3 -0-phosphoramidites on a controUed-pore glass support Synthesis of a 43-nucleotide sequence similar to the 3 -half molecule of Escherichia coli formylmethionine tRNA. J Am Chem Soc 109 7845-7854, 1987. 

Fontain > demonstrates the use of the GA for the problem of calculating chemical distance between molecules for use in automated chemical synthesis planning methods. The essential idea is that a starting material (SM) is transformed through a series of steps into product (P). At each step k, the intermediate can in principle be transformed into a series of new intermediates ijj+j, ijflj,. One of the criteria used to rule out certain of these... 

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