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Atomic force microscopy plasmon

There are several other techniques Uke the fluorescent dye displacement assays, footprinting, Fourier transform infrared spectroscopy. X-ray crystallography, electron microscopy, confocal microscopy, atomic force microscopy, surface plasmon resonance etc used for hgand-DNA interactions that are not discussed here. [Pg.173]

Surfaee plasmon resonanee Needs mobile and immobile phase, atomic force microscopy... [Pg.170]

A number of methods are available for the characterization and examination of SAMs as well as for the observation of the reactions with the immobilized biomolecules. Only some of these methods are mentioned briefly here. These include surface plasmon resonance (SPR) [46], quartz crystal microbalance (QCM) [47,48], ellipsometry [12,49], contact angle measurement [50], infrared spectroscopy (FT-IR) [51,52], Raman spectroscopy [53], scanning tunneling microscopy (STM) [54], atomic force microscopy (AFM) [55,56], sum frequency spectroscopy. X-ray photoelectron spectroscopy (XPS) [57, 58], surface acoustic wave and acoustic plate mode devices, confocal imaging and optical microscopy, low-angle X-ray reflectometry, electrochemical methods [59] and Raster electron microscopy [60]. [Pg.54]

Gobi, K.V., S.J. Kim, H. Tanaka, et al. 2007. Novel surface plasmon resonance (SPR) immunosensor based on monomolecular layer of physically-adsorbed ovalbumin conjugate for detection of 2,4-dichlorophenoxyacetic acid and atomic force microscopy study. Sens. Actual B Chem. 123 583-593. [Pg.172]

One of the advantages of SAMs on smooth, reflective surfaces, is that reactions on these monolayers can be studied by a wide range of techniques including infrared spectroscopy 20 scanning electron microscopy (27), contact angle measurements (22), atomic force microscopy (AFM) (25), surface plasmon... [Pg.182]

Structure determination continues to be an expanding area of research, with the target complexes also becoming more complex as techniques are refined and newer techniques introduced. In addition to X-ray and NMR solution studies there have been many reports concerning structure determination by visualisation using techniques such as electron microscopy, surface plasmon resonance and atomic force microscopy. [Pg.181]

Finally, we note that the predictions from these simulations could be directly probed with surface spectroscopies such as sum frequency generation (SFG) spectroscopy [16]. Provided self-assembly of SAMs of different chain lengths was possible, adsorption of LKo(14, we predict, would reveal no appreciable SFG signal compared to neat SAMs, which reveal the expected helical structures. Likewise, using a combination of techniques such as surface plasmon resonance (SPR) and atomic force microscopy (AFM) [41], we propose it would be possible to study the expected increases in binding energy due to the film formation defects. Of course, this would depend on being able to synthesize in a controlled way the film-type defects. [Pg.34]

X-ray photoelectron spectroscopy Surface plasmon resonance Fluorescence spectroscopy and microscopy (including immunofluorescence, total internal reflection fluorescence) Environmental scanning electron microscopy Atomic force microscopy Fluorescence spectroscopy and microscopy (including immunofluorescence, total internal reflection fluorescence)... [Pg.168]

The stmctural and conformational analysis of proteins adsorbed to solid surfaces is difficult because most common analytical methods are not compatible with the presence of the interacting solids. With recent developments in instrumentation and techniques, our understanding of protein adsorption behavior has improved considerably [4, 14]. The most commonly used techniques include attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), radiolabeling techniques, immunofluorescence enzyme-linked immunosorbent assay (ELISA), ellipsometry, circular dichroism (CD) spectroscopy, surface plasmon resonance (SPR), and amide HX with nuclear magnetic resonance (NMR). Atomic force microscopy (AFM) and scanning... [Pg.266]

Brinen, J.S., Rosati, I,., Chakel, J. and Lindley, P. (1993) The effect of polymer architecture on the SIMS spectra of glvcolide / trimethylene carbonate copolymers. Surface and Interface. Analysis, 20, 105.5-1060. Burnham,. kA. and Colton, R.J. (1993) Scanning Tunneling Microscopy and Specuoscopy Theory, Techniques and Applications. Force microscopy, ed. Bonnell D.A., New York, VCl I Publishers Inc. Chen, X., Shakesheff, KM., Davies, M.C., Heller, J., Roberts, C.J., Tendler, S.J.B, and Williams, P.M. (1995) The degradation of a thin polymer film studied by simultaneous in situ atomic force microscopy and surface plasmon resonance analysis. Journal of Physical Chemistry, 11, 2547. [Pg.450]

ShakeshefE K.M., Chen, X., Datles, M.C., Domb, A., Roberts, C.J., Tendler. SJ.B. and Williams, P.M. (1995) Relating the phase morphology of a biodegradable polyaner blend to ero.sion kinetics using simultaneous in situ atomic force microscopy and surface plasmon resonance analysis., Langmuir, 11, 3921-3927. [Pg.452]


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