Assays yeast oestrogen assay

Vanderperren, E. Demare, W. Blust, R. Cooreman, K. Bossier, P. Oestrogenic activity of CPRG (chlorophenol red- 3-D-galactopyranoside), a 3-galactosidase substrate commonly used in recombinant yeast oestrogenic assays. Biomarkers 2001, 6, 375-380. 

Studies investigating hormonal activity revealed the oestrogenic activity of short-chain NPE in a number of test systems using either recombinant yeast, oestrogen-sensitive MCF-7 cells [98] or a rodent uterotrophic assay response. None of these assays have yet been validated as an internationally accepted toxicity test method, although the MCF-7 and uterotrophic assays have been established for a number of years as standard assays for oestrogenic activity. 

Commercial DDT and DDD contain o,p-DDT [ 1,1,1-trichloro-2-(c -chlorophenyl)-2-(p-chlorophenyl)] ethane and o,p-DDD [l,l-dichloro-2-(o-chlorophenyl)-2-(p-chlorophenyl)] ethane isomers ( 10-20%), which are chiral [28]. Investigations have demonstrated the oestrogenic activity of o,p-DDT in fish and mammals [29-34]. It is also known that the —)-o,p-DDT enantiomer is a more active oestrogen mimic than the (+)-enantiomer in rats [35, 36]. Subsequently, Hoekstra etal. [37] reported a yeast-based assay to assess the enantiomer-specific transcriptional activity of DDT with... 

Hepatotoxicity—enzyme release and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazodium bromide (MTT) assay on fresh or cryopreserved (human) hepatocytes Endocrine disruption—yeast and mammalian reporter gene assay (oestrogenicity and adrogenicity)... 

Gaido, K.W., Barlow, K.D. and Leonard, L. Use of a yeast-based oestrogen receptor assay to assess chemical interactions with the oestrogen receptor, SOT 1996 Annual Meeting, Abstract 731 cited in Fundamental and Applied Toxicology, Supplement, The Toxicologist, 30(1), Part 2, March 1996. 

---