Array cell-signalling

Soen Y, Mori A, Palmer TD, Brown PO (2006) Exploring the regulation of human neural precursor cell differentiation using arrays of signaling microenvironments. Mol Syst Biol, doi 10.1038/msb4100076... 

At present it is not known if all H3 receptor isoforms similarly activate the wide array of signaling pathways. In transfected cells, the rat H3(413) and H3(397) isoforms both inhibit adenylate cyclase more efficiently than does the hill length rat H3 receptor isoform (445 amino acids), but the former are less efficient in activating the MAPK pathway. Much less information is available for the human H3 receptor isoforms but shorter isoforms seem to couple more efficiently to G -proteins to inhibit cAMP production in transfected cells [40],... 

Genomic tools 31 This method takes advantage of select genomic (microarray-or PCR-based approaches) and proteomic (antibody array analysis) tools to identify the receptors for growth factors, hormones, cytokines, and other components of cell signaling pathways expressed by a culture of interest. 

Comparison of multiple phosphoproteomic endpoints for a group of samples on the array permits evaluation of the interconnected cell-signaling proteins in the sample populations. 

Senaratne W, et al. Functionalized surface arrays for spatial targeting of immune cell signaling. J Am Chem Soc 2006 128(17) 5594—5. 

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