PDE-deficient yeast

FIGURE 5.5 Yeast-based screen for inhibitors of human PDE IV. A PDE-deficient yeast (PMY) will not utilize acetate as a sole carbon source and is sensitive to heat shock (55°C). Complementation with a human type IV PDE (PMY + PDE) expressed from a copper-dependent (CUPl) promoter reverses the mutant phenotype. Addition of type IV PDE inhibitor (rolipram) to the complemented yeast restores the mutant phenotype (PMY + PDE + rolipram). 

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