Aquatic plant tests

Four bioassays a) 30 min acute bacterial test (Vibrio fischeri) b) 8d growth rate aquatic plant test (Lemna minor) c) 72h germination plant test (Lepidium sativum) and 8d root elongation plant test (Lepidium sativum) d) 72h germination plant test (Brassica rapa) and 8d root elongation plant test (Brassica rapa) Municipal and industrial solid waste leachates originating from landfills Unspecified pretreatment... 

However where classification is applied solely due to the acute toxicity (L(E)C5o) observed in single algae/aquatic plant tests, but there is evidence from a range of other algae tests that the chronic toxicity (NOECs) for this taxonomic group is above lmg/1, this evidence could be used to consider declassification. At present this approach cannot be applied to aquatic plants since no standardized chronic toxicity tests have been developed. 

A relatively small number of studies have reported on the effects of cumene on plants, fish, and other organisms. Studies of the effects of cumene on fresh and saltwater fish indicate the lowest reported toxic concentration (LC q) for fishes was 20 to 30 mg/L (18). The solubiUty of cumene is about 50 mg/L (19). Among invertebrates, the lowest reported concentration that was toxic to test organisms was 0.012 mg/L after 18 hours (20). The only available data on the effect of cumene on aquatic plants indicate that the photosynthesis of several species was inhibited at concentrations from 9 to 21 mg/L (19). 

Studies on phytotoxicity of bibenzyl derivatives were extended to duckweed axenic cultures. Bibenzyls 19 and 20 as well as the synthetic analogs 23—28 were tested by triplicate in cultures of the small aquatic plant. Analogs 23—28 were synthesized to investigate the effect on phytotoxicity of oxygenated substituents (phenolic vs. phenolic methyl ether) and their location on the bibenzyl core structure. All synthesized analogs but... 

No studies on the acute toxicity of C12LAS to algae and aquatic plants were found in the literature. Typical toxicity-testing protocols use a test duration of 4-7 days. This duration of testing represents a significant portion of the organisms life span for the taxa tested. Thus these toxicity tests are appropriately considered chronic-toxicity tests. 

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