Necrosis, apoptosis comparison

Studies in various animal models and in human hearts suggest that apoptosis does occur in ischemia/reperfusion injury of the heart, though the relative contribution of apoptosis in comparison with necrosis to cell loss in ischemia/ reperfusion injury is still controversial. Cardiomyocyte apoptosis was first reported by Gottlieb et al. [107], who studied the ischemia/reperfusion in rabbit hearts and found the hallmark of apoptosis in ischemic/reperfused hearts but not in the normal or ischemic-only rabbit hearts. Identification of apoptosis was based on the presence of fragmented DNA in electrophoretic gels, on in situ nick end-labeling assays, and on electron microscopy. They concluded that apoptosis may be a specific feature of reperfusion injury in cardiac myocytes. Subsequent studies have shown that apoptosis probably occurs both in ischemia and reperfusion [108], It appears that apoptosis is more prominent after ischemia followed by reperfusion than after ischemia alone [109, 110],... 

Lizard, G., Monier, S., Cordelet, C., Gesquiere, L., Deckert, V., Gueldry, S., Lagrost, L., and Gambert, P., 1999, Characterization and comparison of the mode ofceU death, apoptosis versus necrosis, induced by 7beta-hydroxycholesterol and 7-ketocholesterol in the cells of the vascular wall, Arten oicZer. Thromb. Vase. Biol. 19 1190-1200. 

Necrosis Necrosis is a cell s response to overwhelming injury. It has been referred to as accidental cell death by way of comparison with apoptosis, which is considered to be a more orderly and beneficial process. The flow cytometric hallmark of necrosis is permeabilization of the outer membrane (leading to propidium iodide fluorescence). 

The benzyl-substituted titanocene 3, which showed a significant higher cytotoxicity when tested in vitro against LLC-PK, was evaluated in a series of biomedical studies as well. Watson et al. tested titanocene 3 in comparison to two aryl-substituted tf .va-titanocenes on prostate cancer cells, as advanced prostate cancer is not curable up to now [37]. Therefore, it was shown that all three titanocenes induced more apoptosis (programmed cell death, in contrast to necrosis) compared with cisplatin in a dose-dependent manner. Titanocene 3 had the most significant effect on the cell cycle and apoptosis. 

TABLE 16.2. Comparison of Morphological Features of Apoptosis and Necrosis... 

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