Antiserum inhibition

Figure 9. Anti-PbTx antiserum inhibition of [ H]PbTx-3 binding to its receptor site in rat brain membrane preparations. Labeled toxin (0.5 nM in 1 ml PBS) was incubated with rat brain membranes (125 fig total protein) and increasing amounts of anti-PbTx antiserum (- -) or pre-immune serum (- -) for 1 hr at 4 C. Membrane-bound radioactivity was then measured in a centrifugation assay as previously described (8),...

Terai, H. Adenylate kinase from Pseudomonas denitrificans. I. Purification and antiserum inhibition. J. Biochem., 75, 1027-1036 (1974)... 

In order to block the effects of spinally released CGRP, an antiserum against CGRP was tested in several models of chronic pain and was found to be antinociceptive in models of chronic inflammatory pain such as adjuvant-arthritis (Kuraishi et al., 1988) or carrageenan-induced hyperalgesia (Kawamura et al., 1989). In addition this antiserum inhibited the repeated cold stress-induced hyperalgesia in rats (Satoh et al., 1992). 

Vos-Scheperkeuter etal purified a single form of branching activity of 79 kDa molecular mass from potato tubers. The antibodies prepared against the native potato enzyme was found to react strongly with maize BEI and weakly with maize BEIIb. The antiserum inhibited the activities of both potato tuber BE and maize BEI in neutralization tests. Potato BE thus shows a high degree of similarity to maize BEI and a lesser extent with the maize BEII isozymes. 

Antibodies have been raised against 5 -nucleotidase of mouse liver plasma membranes by using purified membrane preparations from the same source (GURD EVANS, 1974) and the antiserum inhibited enzyme activity. Antisera raised against rat liver as well as rat fat cell plasma membranes inhibited ecto-5 -nucleotidase in isolated rat fat cells (NEWBY al., 1975). However, it is not known whether the immune-specific and the catalytic site of the enzyme are identical. 

Increasing amounts of antiserum inhibited the enzyme activity of both normal erythrocytes and of the erythrocytes of the Lesch-Nyhan patient with the partial enzyme deficiency ... 

After initial experiments demonstrating that the antiserum was capable of completely inhibiting the binding of [ H]PbTx-3 to its receptor site in rat brain membranes (Figure 9), we began studies designed to evaluate potential of the antiserum for prophylaxis and treatment of brevetoxin intoxication (34). The tethered rat model was used, and surgical implantations were identical to those described above. Heart rate, core and peripheral body temperatures, lead VIO ECG, and arterial blood pressure were monitored continuously. Respiratory rate was recorded each 5 min for the first 3 hr, then each 15 min until 6 hr. 

The addition of increasing amount of unlabelled morphine to a fixed amount of (3H) dihydromorphine and antiserum results a competitive inhibition of the labelled dihydromorphine for the formation of... 

A strong link between the phosphorylation of nucleolin, its proteolysis and the production of ribosomal RNA has been observed (Bouche et al, 1984 Bourbon et al, 1983 Warrener and Petryshyn, 1991). The inhibition of proteolysis using leupeptin leads to a lower rRNA transcription in an in vitro transcription system (Bouche et al, 1984). In another series of experiments, the injection of nucleolin antiserum leads to 2-3.5 fold stimulation of pre-rRNA synthesis in Chironomus tentans salivary glands (Egyhazi et al, 1988), although it was not clearly demonstrated that these antibodies blocked specifically the homolog of nucleolin in this species. A model was proposed based on these observations where nucleolin was... 

The neutral FDPase and SDPase activities, which were present in the crude spinach extracts, were precipitated at lower ammonium sulfate concentration and could thus be separated from the specific alkaline FDPase. These activities appeared to be associated with the chloroplast fraction and did not require the presence of a divalent cation for activity. In crude extracts only the alkaline FDPase activity was inhibited by antiserum prepared by immunizing rabbits with the purified alkaline FDPase. The neutral FDPase was also active with ribulose diphosphate (RuDP) (98). 

The specificity of the antiserum raised against the Lea neoglycoprotein (9) was determined by hapten-inhibition studies using synthetic oligosaccharides,72 and derivatives of bovine and horse serum-albumin to which the Lea trisaccharide had been attached.73 On the basis of hapten inhibition of precipitation, this antiserum was shown to be highly specific for the carbohydrate portion of the synthetic antigen,... 

Fig. 1.—Hapten Inhibition of the Precipitation of the Synthetic Antigen 8-Carboxy-octyl 4-Chloro-4-deoxy-0-D-galactopyranoside-BSA by Rabbit Antiserum Raised Against 8-Carboxyoctyl 0-D-Galactopyranoside-BSA (12 in Table I). [The haptens were the oj-earboxyalkyl /3-D-galactopyranosides having the aglycons (1) 8-carboxyoctyl, (2) 4-carboxybutyl, (3) 2-carboxyethyl, and (4) carboxymethyl (taken from Ref. 73).]...

Figure 7. Specific binding of Sendai virus to GQlb ganglioside and its inhibition by antiserum as demonstrated with (a) the VCS method and (b) hemadsorption (see text). A virus in fourfold serial dilutions in buffer B in lower-titer antiserum, 1% C in the same serum, 10% D in higher titer antiserum, 1% this serum, 10%.

Antiserum at 37°, 15 min Wash 0°. +Lectin 100-300 yg/ml at 0°, 30 min Wash, 0° Rabbit anti-lectin serum, 0°, 15 min Wash 0° Fluorescein labelled goat antirabbit IgG, 0°, 15 min Wash 0° View. FITC, Fluorescein isothiocyanate. TABLE II. Inhibition of Immunofluorescence ... 

Chemical inhibition with CYP-selective direct-acting and metabolism-dependent inhibitors and antibody inhibition with CYP-selective polyclonal or monoclonal antibodies (antiserum, ascites fluid or purified antibody)... 

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