Purification antigenic vaccine protein

A. Purification of an Antigenic Vaccine Protein by Selective Displacement Chromatography... 

Shukla AA, Hopfer RL, Chakravarti DN, Bortell E, Cramer SM. Purification of an antigenic vaccine protein by selective displacement chromatography. Biotech Prog 1998 14 92-101. 

Proteins such as antibodies, enzymes, hormones and vaccine antigens can be used to prevent, diagnose and treat a range of diseases. Such molecules are therefore of paramount importance in health and medicine. Historically, many of these proteins have been isolated from human or animal sources. However, the low quantities present in such source material coupled with safety risks and high purification costs have limited the availability of protein therapeutics and vaccines for many types of disease. 

Several plant vims coat proteins, including those of TMV, CPMV, AlMV and Tomato bushy stunt vims (TBSV), have been used to produce and deliver antigenic determinants from a variety of viral and bacterial pathogens. These data have been summarized in numerous publications and several reviews [12,13]. The ease of virus purification coupled with enhanced peptide immunogenicity when fused to carrier molecules makes this approach very attractive for vaccine development. 

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