Antibody, antigen binding fragment

Figure 1.77 Disulfide reducing agents such as 2-mercaptoethylamine can be used to cleave the disulfide bonds in the hinge region of antibody molecules. Either intact IgG molecules or F(ab )2 fragments may be reduced in this manner to yield monofunctional antigen binding fragments.

Fig. 4-8 Stylized representations of protein structures in which a helices are represented as coiled ribbons and p strands are represented by arrows pointing in the N — C direction, p proteins contain predominantly /3-sheet structure (e.g., retinol binding protein and the antigen binding fragment of antibodies) while a proteins contain predominantly a helices (e.g., myoglobin), alp proteins contain a mixture of a helix and p sheet (e.g., triosephosphate isomerase).

Incorporation of (111) into a kinetically stable complex suitable for attachment to an antibody calls for an octadentate ligand, and so pendant-arm cyclen derivatives are appropriate. In particular, two or three antigen-binding fragments of an antibody (Fabs) have been attached to the di- or trimaleimide ligands 80-82 (Scheme 8) (90, 91)... 

Clezardin, P., McGregorJ. L., Manach, M.,Boukerche, H., arid Dechavanne, M (1985) One-step procedure for the rapid isolatiort of mouse monoclonal antibodies and their antigen binding fragments by fast protein liquid chromatography on a Mono-Q anion exchange column./ Chromatogmphy S19, b - . 

Sutherland, R. M., Buchegger, F Schreyer, M Vacca, A., and Mach, J. P. (1987) Penetration and binding of radiolabeled anti-carcinoembryonic antigen monoclonal antibodies and their antigen binding fragments in human colon multicellular spheroids. Cancer Res. 47, 1627-1633. 

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