Anticoagulated whole blood, platelet

In summary, the capillary perfusion system represents a simple in vitrc technique for the quantitation of protein adsorption and platelet accumulation on artificial surfaces under well defined hydrodynamic conditions (0-4.,000 s ). Radioisotopic methods enable simultaneous study of platelet deposition and adsorption of one or more plasma proteins. Experiments may be performed using washed platelet suspensions or anticoagulated whole blood, platelet deposition being measured in the latter case by surface phase radioimmunoassay with a... 

Isolation of Human Platelets The steps described in the subsequent subheading outline the procedure for isolation and purification of platelets from whole blood obtained by venipuncture from human volunteers. Obtain blood sample by venipuncture from an antecubital vein into polypropylene syringes containing either sodium citrate (0.38 % final concentration) or heparin (10 U/ml final concentration). Centrifuge anticoagulated whole blood at 160 x g for 15 min to prepare platelet-rich plasma (PRP). 

Incubate anticoagulated whole blood with platelet antagonist or vehicle (control) at 37 °C for 10 min. 

Table IV. Platelet Accumulation from Heparin Anticoagulated Whole Blood on Glass Capillary Surfaces Precoated v/ith Purified Human Albumin, Hnman Fibrinogen or Bovine Collagen. Quantitaticr of Platelet Deposition by Badioimmuroassay with Iodine Labeled Monoclonal Antibody 6C9 against Human Platelet Glycoprotein Complex Ilb-IIIa...

Serotonin can be measured in whole blood, serum, platelet-rich plasma, platelet-poor plasma (i.e., platelet-free plasma), isolated platelet pellets, urine, and CSF. Most blood serotonin is stored in the platelets and is easily released during sample preparation. For whole blood serotonin, venous blood (10 mL) is drawn into a tube containing potassium EDTA as an anticoagulant, gently mixed, placed on ice, and transferred to a storage tube. An aliquot of blood is then removed for a platelet count alternatively, a simultaneous EDTA blood sample can be collected. Antioxidants such as ascorbic acid or metabisulfite are conveniently added to the storage tube when a fluorometric detection system is used. Blood serotonin samples are stored frozen at -20 °C, preferably within 2 hours after collection. 

Sokolov A, Hellerud BC, Tonnessen TI, Johannessen EA, Mollnes TE. Activation of coagulation and platelets by candidate membranes of implantable devices in a whole blood model without soluble anticoagulant. Journal of Biomedical Materials Research PartA2013 101(2) 575-81. 

Many methods are available to measure platelet a egation in vitro. First, the blood sample is treated with an anticoagulant to avoid clotting of the blood in the test tube or in the aggregometer many different anticoagulants are used, which all differ in their mechanism of action. Second, platelet aggregation can be measured in whole blood, in platelet-rich plasma, or (to remove the influence of the... 

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