Antibodies for Immunohistochemistry

Cuello AC, Milstein C, Galfre G. 1983. Preparation and application of monoclonal antibodies for immunohistochemistry and immunocytochemistry. Immunohistochemistry, Cuello AC, editor. Chichester John Wiley Sons. 

To further assess the clinical relevance of a particular cancer target it may be necessary to determine the association of the target protein levels, its localization, and/or its activity with a specific cancer type, stage, or prognosis. This will often require the generation and, most importantly, validation of robust antibodies for immunohistochemistry (IHC) to be used on tissue microarrays (TMAs) encompassing the relevant tumor types and, ideally, matched normal samples. 

Baas, I., Mulder, J., Offerhaus, G., Vogelstein, B., and Hamilton, 5. (1994) An evaluation of six antibodies for immunohistochemistry of mutant p53 gene product in archival colorectal neoplasmas. J. Pathol. 172, 5-12. 

Availability of Human Reactive Bcl-2 Family Antibodies for Immunohistochemistry... 

These methods may break down crosslinks between the antigen and the surrounding proteins, allowing the epitopes to become accessible due to the mechanism(s) responsible for the availability of epitopes for immunohistochemistry—a strategy to ensure that recognition of the epitope by the antibody is required. Such a protocol is presented below. 

Campbell, E., Pearson, R. C. A., and Parkinson, D. 1999. Methods to uncover an antibody epitope in the KPI domain of Alzheimer s amyloid precursor protein for immunohistochemistry in human brain. J. Neurosci. Methods 93 133-138. 

Elucidation of the primary structure of PBAN opened up a new field of research with immense possibilities. The exact site and the specific neurosecretory cells that produce PBAN need to be identified. We also need to determine where, when, and how PBAN is released. Development of antibodies for their use in immunohistochemistry should prove useful. A radioimmunoassay for PBAN will be useful to more precisely detect the hormone in various tissues such as hemolymph, and follow the time-course of release and determine PBAN titer at various times of the day. Identification of the receptors for PBAN is crucial to the study of its mechanism of action (how does PBAN induce pheromone production ). 

Tujague, M., D. Saligaut, C. Teitsma, O. Kah, Y. Valotaire and B. Ducouret. Rainbow trout glucocorticoid receptor overexpression in Escherichia colt production of antibodies for Western blotting and immunohistochemistry. Gen. Comp. Endocrinol. 110 201—211, 1998. 

FIGURE 1.11 Schematic of polymer-based detection system. The enzyme-linker antibody has a more compact molecular shape than other polymer carrier-based conjugates and thus allows the attachment of multiple conjugates in close proximity to each other. The abundant conjugated enzymatic molecules are deposited in each antigenic location, which resembles a big-city skyline. From Shi S-R, Quo j, Cote R], et al. Sensitivity and detection efficiency of a novei two-step detection system (PowerVision) for immunohistochemistry. Appi immunohistochem Moi Morphoi. 1999 7 20i-208. 

The overwhelming majority of neuroendocrine neoplasms occurring in the liver are metastatic. Well to moderately differentiated neuroendocrine neoplasms (e.g., carcinoids, pancreatic endocrine neoplasms, medullary thyroid carcinomas) may mimic primary hepatocellular processes, and thus immunohistochemistry with antibodies for chromogranin, synaptophysin, and CD56 can be extremely helpful, as discussed earlier. They may also be positive for MOC-31 but not for Hep Par-1. In contrast, HCC may occasionally show focal neuroendocrine differentiation and may stain for synaptophysin. 

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