Antibodies anti platelet binding

Christie DJ, Mullen PC, Aster RH (1985) Fab-mediated binding of drug-dependent antibodies to platelets in quinidine- and quinine-induced thrombocytopenia. J Clin Invest 75 310-314 Cunha PD, Lord RS, Johnson ST, Wilker PR, Aster RH, Bougie DW (2000) Immune hemolytic anemia caused by sensitivity to a metabolite of etodolac, a nonsteroidal anti-inflammatory drag. Transfusion 40 663-668... 

ELISA) in which the antibodies bind to a target lipid, which is usually cardiolipin. Platelet antibodies directed to phosphatidylserine and phosphatidylinositol are referred to as anticardiolipin antibodies and their presence is characterized as anti-cardiolipin antibody-thrombosis syndrome (96). 

Peerschke EIB. Adhesive protein expression on thrombin-stimulated platelets time dependent modulation of anti-fibrinogen, -fibronectin, and -von Willd>rand Factca- antibody binding. Blood 1992 79 2028-2033. 

Selectins include P-selectin (platelet selectin), E-selectin (endothelial cell selectin), and L-selectin (leukocyte selectin). P-selectin enables binding of platelets, polymorphonuclear leukocytes, and monocytes to activated endothelial cells and of leukocytes to activated platelets. P-selectin is expressed in the kidneys in systemic lupus erythematosis [266, 267]. The up-regulation of P-selectin expression in glomerui following binding of anti-GBM antibody may be an... 

The rank order of binding of anti-fibrinogen antibodies, when the series of methacrylates was pre-exposed to fibrinogen solution, diluted plasma, or to whole plasma (for short times), showed significant positive correlations with the measurements of platelet reactivity induced by these polymers (Fig. 6). These correlations suggest that platelet activation induced by these polymers may depend upon the availability of conformationally unaltered, "native fibrinogen on the surface, and may be less dependent on the total amount of bound fibrinogen. 

Figure 6. Correlation of anti-fibrinogen antibody binding with platelet activation by polyalkyl methacrylates. Polymer-coated glass cover slips were exposed first to fibrinogen solution (250 ug/ml) and then to 1 25x-labeled antibody solution (300 ng/ml, 800 cpm/ng). (Reproduced with permission from Ref. 24. Copyright 1986 Grune Stratton.)...

Reagent for enzyme-linked immunoassay Isolation by immunoadsorption of immunoglobulin G Fc fragment-binding glycoproteins from human blood platelets Reagent for enzyme immunoassay Reagent for enzyme immunoassay Purification by immunoadsorption of anti-carbohydrate antibodies... 

Also in support of the location of cathepsin M on the outer lysosomal surface were results obtained with a monoclonal antibody prepared against the rabbit liver enzyme. This antibody, but not another monoclonal antibody specific for platelet surface markers, could be shown to bind to the intact lysosomes. In addition, rabbit liver lysosomes could be shown to bind to monoclonal anti-cathepsin M coupled to Sepharose 4B, but not to Sepharose 4B alone or to a coupled monoclonal antibody directed against surface glycoprotein of human platelets (Table II). 

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