Platelet thrombin stimulated

GEA-3175 is more stable than GEA-3162 in vitro but still retains its biological activity [95]. The release of NO and NO2 by GEA 3175 was increased 140-fold in the presence of human plasma, as analyzed by ozone chemiluminescence [94]. GEA 3175 inhibited agonist-induced platelet aggregation and induced a more than 4-fold increase in platelet cGMP without affecting cAMP levels [94]. Thrombin-stimulated rises in the cytosolic free Ca2+ concentration and secretion were dose-dependently inhibited by GEA 3175. GEA 3175 showed a reduced capacity to inhibit platelet aggregation of uremic platelets compared to controls [96]. 

N5. Nierodjik, M. L., Plotkin, A., Kajuno, F., and Karpatkin, S., Thrombin stimulates tumor-platelet adhesion in vitro and metastasis in vivo. J. Clin. Invest. 87, 229-236 (1991). 

Platelets aggregation stimulation. Fruit juice, administered intravenously by infusion to dogs at a dose of 5 mL/min, was active. Total infusion was 300 mL h Oil, administered orally to six New Zealand white rabbits fed a commercial diet supplemented with 60 g/kg of coconut oil low in all PUFA for 60 days, produced a platelets aggregation induced by both thrombin and collagen significantly lower with either fish or linseed oil (n-3 PUFA), than with corn oil (n-6 PUFA) or the low PUFA coconut oil . 

Binding to aggregin is required for epinephrine-induced platelet aggregation to take place. In turn, epinephrine increases the affinity of ADP for its receptor. Thrombin stimulates platelet aggregation independent of ADP, but by raising the level of calcium in the cytoplasm, it activates platelet calpain, which in turn cleaves aggregin (Figure 4.1). 

Table 1 IC50 values for selected five-substituted benzimidazolone peptidomimetics in a thrombin receptor binding assay versus IC50 values for platelet aggregation stimulated by thrombin. In all cases, amino acids have and L-absolute configuration. Ll NMR data supplied by author...

Differential analysis of phosphorylated proteins in resting and thrombin-stimulated human platelets. Anal. Bioanal. Chem. 376, 973-993. 

Marcus, K., Moebius, )., Meyer, H. E. (2003). Differential analysis of phosphorylated proteins in resting and thrombin-stimulated human platelets. Anal. Bioanal. Chem. 276, 973-993. 

Naka, M., Saitoh, M., Hidaka, H. (1988). Two phosphorylated forms of myosin in thrombin-stimulated platelets. 

Hung DT, WongYH, VuT-KH, Coughlin SR. Uie cloned platelet thrombin receptca couples to at least two distinct effectors to stimulate phosphoinositide hydrolysis and inhibit adenylyl cydase. J Biol Chem 1992 267 20831-4... 

Hirao A, Ifomagnciii L Suda T, Yamaguchi N Translocation of the CA homologous kinase (C3ik/Hyl) controls activity of CD36-anchored Lyn tyrosine kinase in thrombin-stimulated platelets. EMBO J 16 2342-2351,1997... 

Silicic DD, Wencd-Drake ID, Parise LV. Ras activation in platelets after stimulation of the thrombin receptor, thromboxane A2 receptor or protein kinase C. Biodiem J1997 321 S2S-S30. 

Peerschke EIB. Adhesive protein expression on thrombin-stimulated platelets time dependent modulation of anti-fibrinogen, -fibronectin, and -von Willd>rand Factca- antibody binding. Blood 1992 79 2028-2033. 

Borsch-Haubold AG, Kramer RM, Watson SP. Cytosolic phospholipase Aj is phosphorylated in collagen- and thrombin-stimulated human platelets independent of protein kinase C and mitogen-activated protein kinase. JBiol Chem 270 25885-25892,1995. 

Brass LF, LAPOSATA M, Banga HS, RTTTENHOUSE SE. Regulation of the phosphoinositide hydrolysis pathway in thrombin-stimulated platelets by pertussis toxin-sensitive guanine nudeotide-binding protein Evaluation of its contribution to platdet activation and comparisms with the adenylate cydase inhibitory protein, Gl. JBiol Chem 261 16838-16847,1986. 

JUNGITW, SPOCHER mo, NYDEGGER UE, BaRANDUN S. Platdd-Ieukocyte interaction selective binding of thrombin-stimulated platelets to human monocytes, polymorphoimdear leukocytes and related cell lines. Blood 661 629-636,1986. 

NAKASHMA S, TOHMATSU T, HATTORI H, OKANO Y, NOZAWA Y. Inhibitory action of cyclic GMP on secretion, polyphosphoinositide hydrolysis and calcium mobilization in thrombin-stimulated human platelets. Biochem Biophys Res Commun 135 1099-1104,1986. 

WHIIE JG, KRUMWMK M Further studies of the secretoiy pathway in thrombin-stimulated human platelets. Blood 69 1196-1203,1987a... 

Phospholipase C (both p and y) catalyzes the hydrolysis of Phosphatidyl inositol bisphosphate (PIPj) leading to the generation of diacylglycerol (DAG) and inositol trisphosphate (IP,). In platelets, thrombin and thromboxane-A, (TxA,) stimulate PLC-p. PLC-P has three isotypes. It is known that PLC-pi and PLC-P3 are activated by G-protein... 

BRASS LF, LAPOSATA M, BANGA HS, RTITENHOUSE SE (1986) Relation of the phosjAoinositide hydrolysis pathway in thrombin-stimulated platelets by a pertussis toxin-senative guanine nucleotide-binding protein. Evaluation of its contribution to platelet activation and comparisons with the adenylate cyclase inhibitory protein, Gi. Journal of Biological Chemistry, 261,16838-16847. 

Mahadevappa VG, and Holub BJ. (1987). Quantitative loss of individual eicosapentaenoyl- relative to aradiidonoyl-containing pho diolipids in thrombin-stimulated human platelets. J. Upid Res. 28, 1275-1280. 

Mahadevappa VG, and Holub BJ. (1986). Diglyceride pathway is a minor source o( released arachidonic acid in thrombin-stimulated platelets. Biochem. Biophys. Res. Commun. 134,1327-1333. 

Mahadevappa VG, and Holub BJ. (1983). Degradation of different mdecularspedes of idnsphatidyl-inoshol in thrombin stimulated human platelets Evidence for preferential deradation of 1-acyl 2-aradiidonoyl species. J. Biol. Chem. 258,5337-5339. 

Zavoico GB, Halenda SP, Sha afi RI, and Feinstein MB. (1983). Phorbol myristate acetate irdiibits thrombin-stimulated Ca mobilization and phosphatidylinoshol 4, 3-bisphoq>hate hydrcdysis in human platelets. Proc. Natl. Acad. Sci. U.S.A. 82,3839-3862. 

Kramer RM, Roberts EF, Um SL, Borsch-Haubold AO, Watson SP, Fisher MJ, and Jakubowski JA (1996). p38 mitogen-activated protein kinase jdiosphorylates cytosolic phospholipase A2 (cPLA2) in thrombin-stimulated platelets. Evidence that proline-directed phosph lation is not required ftn-mobilization of arachidonic acid by cPLA2. J. Biol. Chem. 271,27723-27729. 

Nsicasfaiina S, Tohmatsu T, Hattori H, Okano Y, Nozawa Y (198Q Inhibitory actiem of cyclic GMP on secrelim, phoqphnnositide fayifcafyais and calami mobilization in thrombin-stimulated human platelets. Biodiem Biophys ResCommun 135 1099-1104. 

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