Antibodies, anti-thymidine

Fig.I. Specificity of antinucleoside and antinucleotide antibodies. (A) Inhibition of the precipitation of antiadenosine antibodies and adenosine-serum albumin by adenosine (O), deoxyadenosine ( ), adenosine 5 -monophosphate (A), adenine ( ), and cytidine, thymidine, or guanosine (+). (B) Inhibition of the precipitation of anti-GMP antibodies and GMP-serum albumin by GMP ( ), dGMP ( ), and guanosine (x).

Kelley, M., Cooper, C., Matticoli, A., and Greway, A. (2005) The detection of anti erythropoietin antibodies in human serum and plasma. Part II. Validation of a semi quantitative 3H thymidine uptake assay for neutralizing antibodies. Journal oflmmuno logical Methods, 300, 179 191. 

BrdU 5-Bromodeoxyuridine (also abbreviated BUdR or BrdUrd) is a thymidine analog that will be incorporated into the DNA of cycling cells. Cells pulsed with BrdU can then be stained with anti-BrdU monoclonal antibodies to indicate which cells have been synthesizing DNA during the pulse period. BrdU staining is a more precise way to look at the proportion of cells in S phase than simple propidium iodide staining for DNA content. 

A promising approach is the monoclonal antibody conjugate targeted PDT such as conjugates to anti-EGF or conjugates to anti-transferrin receptor [85,86] or the targeted therapy against the thymidin kinase system [6]. 

---