Anti-carbohydrate antibodies glucuronic acid

Fig. 35.—Agar diffusion of xanthan antibodies (Ab) and chemically modified xanthan and inhibitors. A xanthan (well 1) reduced xanthan (well 2) oxidized xanthan (well 3) deacety-lated xanthan (well 4) enzymatically treated xanthan (well 5). B Xanthan with 6% pyruvate (well 1), xanthan with 4% pyruvate (well 2), xanthan with 2.8% pyruvate (well 3), xanthan with 0.5% pyruvate (well 4), and xanthan with 0.2% pyruvate (well 5). C, D, E Inhibition of anti-xanthan antibodies by glucuronic acid and mannose wells 1-6 contain decreasing concentrations of xanthan (1-0.3%) solutions of xanthan In antibody + glucuronic acid, In2 antibody + mannose. (Reprinted from Carbohydrate Polymers, Volume 27, J. H. Pazur, F. J. Miskiel, and N. T. Marchetti, pp. 85-91, copyright 1995, with permission from Elsevier Science Ltd., The Boulevard, Langford Lane, Kidlington, 0X5 1GB, UK.)...

Quantitative precipitin tests were performed as described.73 The amounts of precipitates obtained at the various concentrations were measured by the protein phenol method.74 The inhibition values are recorded in Table I. These inhibition data show that the precipitin reaction between the tetrahet-eropolysaccharide and the anti-GlcA antibodies is strongly inhibited by d-glucuronic acid, but with the exception of galacturonic acid none of the other carbohydrates were inhibitory. The data in the table also show that the carbohydrates tested did not inhibit the precipitin reaction between the polysaccharide and anti-GlcA-Rha antibodies. The monosaccharides alone cannot completely fit the active site of this antibody to give a precipitin test. 

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