Anti-carbohydrate antibodies characterization

Because of the lipase deficiency, fat-soluble vitamin (A, D, E, and K) deficiencies may occur. Whether lipase activity or bile acids (e.g., in micelle formation) are involved in fat-soluble vitamin absorption with steatorrhea is unclear. Vitamin and zinc deficiencies also may occur as aresult of pancreatic enzyme deficiency. Although pancreatic involvement is predominantly exocrine in nature, insulin deficiency with glucose intolerance also occurs in CF patients, especially as they advance in age. Carbohydrate intolerance is characterized by low insulin concentrations and enhanced peripheral sensitivity to insulin but not by the presence of islet cell or anti-insulin antibodies. Carbohydrate intolerance in CF is not usually associated with the ketosis as commonly occurs in type 1 diabetes. This complication involves an increase in the number of insulin receptors with decreased affinity for insulin. Despite a concomitant increase in tissue affinity for insulin, 8% of CF children over 12 years of age require insulin therapy. 

Formaldehyde cross-linking is used extensively on a commercial basis, and the pore structures of formaldehyde-cottons have been characterized. Formaldehyde cross-linked cellulose appears to have some application as a medium for gel filtration of carbohydrates, and an Escherichia coli lipopolysaccharide cross-linked with glutaraldehyde has been used in the isolation of anti-polysaccharide antibodies. ... 

Roberts G.D., Johnson W.P., Burman S., Anumula K.R., and Carr S.A. (1995), An integrated strategy for structural characterization of the protein and carbohydrate components of monoclonal antibodies application to anti-respiratory syncytial virus MAb, Anal. Chem. 67, 3613-3625. 

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