Anthocyanins chromatographic techniques

A novel pigment has been isolated from the petals of Rosa hybrida with complex chromatographic techniques and the structure was elucidated with spectroscopic methods and high resolution fast-atom bombardement mass spectrometry, lH NMR, and FTIR. Anthocyanins were extracted from 7.9 kg of petals of Rosa hybrida cv. M me Violet with 80 per cent aqueous ACN containing 0.1 per cent TFA. The extract was purified in a Sephadex LH-20 column, and the fraction eluted with 80 per cent ACN was further fractionated in a HP-20 column using water, 15, 20 and 30 per cent ACN as mobile phases. The last fraction was lyophilized and separated by preparative RP-HPLC using an ODS column (50 X 5 cm i.d.). Solvents were 0.5 per cent aqueous TFA (A) and water-methanol... 

Because of their considerable commercial importance, the composition and stability of anthocyanin-rich extracts have also been investigated [264], The SEC and HPLC techniques described above were employed for the analysis of four solid and four liquid commercial anthocyanin-rich extracts. The results of SEC and HPLC measurements are compiled in Table 2.92 and Table 2.93, respectively. The measurements indicated that various chromatographic techniques provide useful information on the quality of commercial anthocyanin-rich extracts and they can be successfully applied for the quality control of this class of products [265],... 

C. Froytog, R. Slimestad and O.M. Andersen, Combination of chromatographic techniques for the preparative isolation of anthocyanins — applied on blackcurrant (Ribes nigrum) fruits. J. Chromatogr.A 825 (1998) 89-95. 

The anthocyanin composition of flowers has also been frequently investigated by chromatographic methods. Acylated anthocyanins and flavonols have been extracted from the flowers of Dendrobium cv. Pompadour and separated by TLC and RP-HPLC techniques. Fresh flowers (approximately. 1 kg) were extracted with 101 of methanol-acetic acid-water (4 1 5, v/v), and the extract was concentrated and analysed by preparative and analytical RP-HPLC using ODS columns (150 X 19 mm i.d. and 250 X 4.6 mm i.d.) at 40°C. The gradient consisted of methanol-water-acetic acid in various volume ratios. Flow rates were 4 and 1 ml/min for preparative and analytical separations, respectively. Cyanidin 3- (6-malonylglucoside)-7,3 -di(6-synapilglucoside) and the demalonyl derivative were detected in the flowers [261],... 

---