Annexins, calcium binding

ALG-2 is the fust calcium-binding protein of the EF-hand family found to be directly involved in apoptosis. ALG-2 is a 22 kDa protein and like the other members of the penta EF-hand family, contains five EF-hands, with only two of them functional. ALG-2 protein is expressed in the brain and eye and was found to be upregulated in various cancer tissues. Several targets have been found, such as proteins AEP, Alix, preflin, and annexins, suggesting a putative role of ALG-2 in apoptosis. 

Annexin V is a human placental anticoagulant protein of molecular weight 35kDa that binds to membranes and lipid bilayers containing phosphatidylserine in the presence of free calcium. Annexin V binding to cell surfaces said to result from transmembrane movement of... 

The main function of the intracellular calcium binding proteins is to modulate cellular events in response to the calcium signal. Analysis of the sequence of many of the intracellular calcium binding proteins has suggested the existence of two distinct families the EF domain family and the annexin fold family. For completeness, we have grouped the remainder of the intracellular calcium binding proteins into a miscellaneous category. 

Membrane Calcium-binding Proteins Annexins and C2 Domain 25... 

MEMBRANE CALCIUM-BINDING PROTEINS ANNEXINS AND C2 DOMAIN... 

The first membrane binding is mediated via multiple calcium binding sites located at the convex side of the disk-shaped annexin core. The N-terminus of the protein that is presumed to harbor the second membrane binding site is buried in the core domain in the absence of Cd , and is supposed to get exposed to the aqueous phase if annexin Al binds to a membrane in the presence of calcium ions (see Fig. 6) [42]. To verify this hypothesis, again SSMs are well suited, since they allow separation of the first and second membrane binding events. N-terminal truncated annexin Al an-... 

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