Anisaldehyde-sulfuric acid

Saturated atmosphere yes Detection/Color anisaldehyd/sulfuric-acid violet-red... 

Bile acids Anisaldehyde/sulfuric acid Variety of spots 4... 

Anisaldehyde-sulfuric acid A 1% anisaldehyde solution in acetic acid (acidified by concentrated sulfuric acid) is sprayed on the plate, which is then heated at 105°C to yield a variety of colors. 

The alcohols were chromatographed on silica gel using various eluent systems. The compounds were detected with the fluorescein-bromine reagent. The phosphomo-lybdenic acid reagent is very sensitive but nonspecific. The anisaldehyde-sulfuric acid reagent is of higher sensitivity and specificity. 

Anilinonaphthalene-t-sulfonk Acid Ammonium Salt Reagent References Anisaldehyde — Sulfuric Acid... 

Note Anisaldehyde — sulfuric acid is a universal reagent for natural products, that makes color differentiation possible [6]. The background acquires a reddish... 

Derivatization reagent Anisaldehyde-sulfuric acid VaniUin-snlfu ric add Vanillin-sulfuric VanilUn-sulfuric add (Autosprayer) add ... 

Derivatization reagent Anisaldehyde-sulfuric acid Anisaldehyde-sulfuric add Vanillin-sulfuric acid Vanillin-sulfuric acid... 

To estimate the content of dihydro-2,5-dihydroxy-6-methyl-4//-pyran-4-one (DDMP) saponin and saponin B in selected pea cultivars, an HPTLC method was developed [35]. Silica gel 60 F254 plates were prewashed with methanol and activated at 100°C prior to development in automated multiple development chamber AMD2 using chloroform-methanol-water (55 37 8, v/v). Densitometry, after postchromato-graphic derivatization with anisaldehyde-sulfuric acid, revealed the prevalence of saponin B over DDMP saponin in all pea cultivars. The identities of the two com-ponnds were determined by coupling HPTLC directly to ESI-MS and additionally by offline MALDI-TOF/TOF-MS by application of a purified extract/matrix mixture onto a standard steel MALDI plate. HPTLC plates intended for MS analysis were developed with a modified developing solvent chloroform-methanol-water (6.5 3.5 0.9, v/v). The analytes were eluted from the plates with 0.1% formic acid-acetonitrile (40 60, v/v) by means of TLC-MS interface (CAMAG) and transferred into the ESI-MS system, which operated in positive-ion mode. 

Silica gel eluted with toluene-acetone-acetic acid (70 30 0.05) or n-butanol— acetic acid-water (65 15 25) and spots identified under short-wave UV light and sprayed further with iodoplatinate and anisaldehyde-sulfuric acid reagent [90]... 

Pushpa et al. described a HPTLC method in which benzene extract of C.forskohlii roots was applied to HPTLC plate. Benzene ethyl acetate (80 20 v/v) was used as the mobile phase and anisaldehyde-sulfuric acid was used as the spray reagent. After developing and spraying, the plate was dried at 100-105 °C. Forskolin gave violet colored spot with Rf value of 0.45 [53]. 

Benzene Ethyl acetate (80 20 v/v) HPTLC plate anisaldehyde-sulfuric acid/ 560 nm 0.45 [53]... 

Forskolin is relatively a nonpolar compound, thus sparingly soluble in water. However, it is also insoluble in petroleum ether and xylene. Thus, it is soluble in solvents like toluene, chloroform, ethanol, and methanol. This property is exploited in the fractionation and isolation of forskolin. Also, forskolin is not very much UV sensitive. Its UV absorption maximum is around 200 nm (Fig. 109.2). Hence, during isolation process, monitoring of the fractions is done usually by TLC. However, TLC is cumbersome, as it involves spraying with either anisaldehyde-sulfuric acid reagent or vanilhn-sulfuric acid reagent and heating [7, 17]. 

The ketoalkenyns cannot be detected in . angustifolia and . purpurea roots. So they are suitable markers for the identification of preparations from . pallida roots. They can be analysed by TLC (silica gel toluene-ethylacetate 7 3 detection anisaldehyde/sulfuric acid) or (better) by HPLC (RP 18 solvent gradient 40-80% acetonitrile/water flow 1.0 ml/min detection 210 nm) (Figs. 7 and 8) [31]. Since they are not stable, they are not suitable for standardization purposes. 

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