Anion-Exchange Chromatography of Proteins

Amino acid Integrated amperometry Ninhydrin method 

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Figure 18 Very-high-speed gradient anion exchange chromatography of proteins. Column 0.46 x 3.5 cm ZipSep AX, 3 p. Eluent Tris-HCl, pH 8.0, operated on a gradient from 0-0.5 M NaCl. Flow rate 2ml/min. Detection UV absorbance at 280 nm. (1) Ribonuclease A, (2) carbonic anhydrase, (3) conalbumin, (4) bovine serum albumin. (Reproduced from Hatch, R. G., J. Chromatogr. Sci., 31, 469,1993. By permission of Preston Publications, A Division of Preston Industries, Inc.)...

Hatch, R. G., Very-high-speed anion-exchange chromatography of proteins using monodisperse nonporous polymer particles, /. Chromatogr. Sci., 31, 469 1993. 

Vanecek, G. Regnier, F.E. Variables in the high-performance anion-exchange chromatography of proteins. Anal. Biochem. 1980,109, 345. 

Polymer-based tentacular materials for anion-exchange chromatography of proteins are offered under the trade name Thermo Scientific Dionex ProPac. The specifications of the two different ProPac anion exchangers currently available are summarized in Table 3.37. 

Polymethacrylate Weak anion exchanger (tertiary amine) ProSwift WAX Dionex Corporation 50x4.6mm 50x 1 mm Ion exchange chromatography of proteins... 

Figure 7.7 Anion Exchange Chromatography of the proteins from the free run juice of grape of cv. Incrocio Manzoni 6.0.13. Column Tricorn Mono Q 5/50 GLM detection at wavelength 280 nm buffer A Tris-HCl 20 mM pH 8.5 buffer B buffer A containing NaCl 1 M gradient program from 0 to 14% of B in 70 min, from 14 to 100% B in 20min and from 100 to 0% B in 5min flow rate 0.6mL/min (Vincenzi et al., 2005)...

Table 2 Examples of Commercial Anion-Exchange Resins for Ion-Exchange Chromatography of Proteins...

In 1958, Sober and Peterson [I] described the use of diethylaminoethyl (DEAE)-derivatized cellulose in the anion-exchange separation of proteins. This hydrophilic matrix overcame many of the problems of the PSDVB-based materials. Shortly thereafter, carboxymethyl-derivatized cellulose was introduced for the cation-exchange chromatography of proteins. Separations achieved using... 

Torres, A. R., Edberg, S.C., and Peterson, E. A., Preparative high-performance liquid chromatography of proteins on an anion exchanger using unfractionated carboxymethyl displacers, /. Chromatogr., 389, 177, 1987. 

Alpert, A. J. and Regnier, F. E., Preparation of a porous microparticulate anion-exchange chromatography support for proteins, /. Chromatogr., 185, 375,1979. 

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