Aniline-phthalate reagent

The column is subsequently washed with about 50 ml of distilled water a drop of the final washings should give a negative test for sugar when applied to a piece of chromato graphic paper and sprayed with aniline phthalate reagent after drying the paper. When this is the case the solution is evaporated in vacuum down to a volume of about 5 ml. 

Fig. 36 Dependence of the area of the chromatogram zone at constant amount of applied substance (1 pg) on the reagent employed, top relief representation, below zone areas projected on one another Iodine vapor reacts least sensitively here, aniline-phthalate most sensitively and the GOD reaction (glucose oxidase reaction) most specifically [2161...

Specific reagents react with a particular class of compound, For example, rhodamine B is often used for visualization of lipids, ninhydrin for amino acids, and aniline phthalate for carbohydrates. 

Question 19 Examples of popular chemical reagents used in chromatography for visualization and detection are ninhydrin (for amino acids), rhodamine B (for lipids) and aniline phthalate (for carbohydrates). 

The reducing methylated sugars are detected by the same reagents used for paper chromatograms such as aniline phthalate (No. 10), p-anisidine phthalate (No. 12), 2,3,5-triphenyltetrazolium chloride (No. 255), alkaline silver nitrate (No. 234) and, in addition, the sulfuric acid sprays and iodine vapor. Brief exposure to iodine vapor does not cause a detectable destruction of the methyl ethers of the sugars [83] and is therefore a convenient method for visualizing the components on preparative plates [73] or for quantitative analysis. 

Some of the commonest reagents, e. g., ninhydrin, bromocresol green, aniline phthalate, are commercially available as aerosol sprayers. These atomise the reagent most efficiently with the simplest operation. 

In another method sugars may be located by applying to the paper chromatogram minute evenly spaced dots of aniline phthalate solution or some other reagent, by means of a multipointed metal stamp (40). 

Partridge, S.M., Aniline hydrogen phthalate as a spraying reagent for chromatography of sugars, Nature, 164, 443, 1949. 

Kowkabany (28) suggests that for identification of the spots the following color reagents have the greatest general usefulness silver nitrate - ammonia, aniline hydrogen phthalate, p-anisidine hydrochloride, o-pheny-lenediamine dihydrochloride, benzidine - acetic acid, 3,5-dinitrosalicylic... 

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