Analytical-scale HPLC separations, residual

Analytical-Scale HPLC Separations. Reverse-phase HPLC chromatography favors the distribution of the semi- and nonpolar constituents of a sample of residue organics, whereas normal-phase HPLC chromatography favors the distribution of semipolar constituents (32). This approach is illustrated in Figure 2 by the chromatograms of residue organics from a waste water sample separated by both reverse-... 

Figure 2. Absorbance (254 nm) profile of the analytical-scale HPLC separation of 25 pg of residue organics isolated from an industrially impacted influent waste water. The separations were accomplished via reverse-phase HPLC (top) and normal-phase HPLC (bottom). Mobile phases used in these separations were water (fyO), acetonitrile (CH3CN)> methylene chloride (MECL), hexane (HX), and isopropyl alcohol (IPROH), as indicated.

Preparative-Scale HPLC Separations. Results of the analytical-scale HPLC separations are used to develop an approach in the scale-up of the HPLC separations for the preparation of subfractions of the residue organics in quantities suitable for mutagenesis testing and compound isolation. If the analytical-scale separation results indicate the... 

Figure 3. Absorbance (254 nm) profile of the analytical-scale reverse-phase HPLC separation of 2.5-L equivalents of residue organics isolated from finished drinking water L...

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