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Analyte-to-matrix ratio

The detection limits and the analyte-to-matrix ratios for inorganic and organic anion impurities in boric acid, obtained using hydrostatic or electro-kinetic injection and with sample stacking (the capillary is filled with the sample up to the detector, a voltage is applied to preconcentrate the sample anions at the sample-buffer interface, a reversed EOF is used to remove the matrix components and... [Pg.1193]

Comparison of limits of detections (LOD) and analyte-to-matrix ratios (ATMR) for inorganic and organic anion impurities in boric acid obtained using hydrostatic and electrokinetic injection and sample stacking [40]... [Pg.1194]

The most common saiiiple-preparalion method is the dried-droplet technique in which a droplet of the matrix containing the analyte is deposited on the metal plate and then dried. Typically, the ratio of analyte to matrix is l l() to 1 10. Analyte concentrations arc usually in the micromolar range. [Pg.560]

We found that target preparation choices such as type and concentration of matrix, the ratio of analyte to matrix, on-target washing, and recrystallization procedures can dramatically affect the quality of resulting MALDI spectra. We usually try two different analyte to matrix dilutions 1-9 pL and -A pL (analyte to matrix) to ensure good-quality spectra. We tune the linear mode method in... [Pg.65]

A general challenge is the analyte-to-matrix molar ratio as many matrices caused interfering mass peaks at low m/z values. High matrix amount decreases selectivity, while a low matrix amount reduces the mass signal intensity and thus detectability. A suitable matrix concentration for UTLC is stated to be 2.66 nmol mm of a-cyano-4-hydroxycinnamic acid, which is about 10 times less than compared to HPTLC (22.3 nmol mm ) [6]. [Pg.161]

Furthermore, the extent to which we can effect a separation depends on the distribution ratio of each species in the sample. To separate an analyte from its matrix, its distribution ratio must be significantly greater than that for all other components in the matrix. When the analyte s distribution ratio is similar to that of another species, then a separation becomes impossible. For example, let s assume that an analyte. A, and a matrix interferent, I, have distribution ratios of 5 and 0.5, respectively. In an attempt to separate the analyte from its matrix, a simple liquid-liquid extraction is carried out using equal volumes of sample and a suitable extraction solvent. Following the treatment outlined in Chapter 7, it is easy to show that a single extraction removes approximately 83% of the analyte and 33% of the interferent. Although it is possible to remove 99% of A with three extractions, 70% of I is also removed. In fact, there is no practical combination of number of extractions or volume ratio of sample and extracting phases that produce an acceptable separation of the analyte and interferent by a simple liquid-liquid extraction. [Pg.544]

The peak area of the unknown (Ax) relative to the peak area of the internal standard (A ) is obtained. Conversion of the measured ratio to a concentration is achieved by comparing it to area ratios of the solutions of known analyte concentration, to which the same quantity of internal standard has been added. A graph of the ratio of the peak area of the component to be measured (A ) to the peak area of the internal standard (Ais) versus the ratio of the weight of the component to be measured (W ) to the weight of the internal standard (Wu.) for the known solutions results in a graph from which the concentration of the component(s) in the unknown matrix (Ax) can be determined (Figure 3.1). [Pg.26]

Mass spectrometry involves the study of ions in the vapour phase. Mass spectrometers are analytical instruments that convert neutral molecules into gaseous ions and separate those ions according to the ratio of their mass-to-charge (m/z) The location of the mass lines provides a qualitative analysis, and their intensity, mostly measured relative to that of the matrix element or a suitable internal standard, gives a quantitative analysis. [Pg.349]

In general, non-spectral matrix issues appear to be a result of space charge effects, where for example, the sensitivity of an analyte decreases as the mass to charge ratio of a matrix element increases (Gillson et al. 1988 Praphairaksit and Houk 2000a). [Pg.137]

The FAB plasma provides conditions that allow to ionize molecules by either loss or addition of an electron to form positive molecular ions, M" , [52,89] or negative molecular ions, M, respectively. Alternatively, protonation or deprotonation may result in [Mh-H]" or [M-H] quasimolecular ions. Their occurrence is determined by the respective basicity or acidity of analyte and matrix. Cationization, preferably with alkali metal ions, is also frequently observed. Often, [Mh-H]" ions are accompanied by [MH-Na]" and [Mh-K]" ions as already noted with FD-MS (Chap. 8.5.7). Furthermore, it is not unusual to observe and [Mh-H]" ions in the same FAB spectmm. [52] In case of simple aromatic amines, for example, the peak intensity ratio M 7[Mh-H] increases as the ionization energy of the substrate decreases, whereas 4-substituted benzophenones show preferential formation of [Mh-H]" ions, regardless of the nature of the substituents. [90] It can be assumed that protonation is initiated when the benzophenone carbonyl groups form hydrogen bonds with the matrix. [Pg.389]

Two somewhat different types of null hypotheses are tested, one during the development and validation of an analytical method and the other each time the method is used for one purpose or another. They are stated here in general form but they can be made suitably specific for experimentation and testing after review and specification of the physical, chemical and biochemical properties of the analyte, the matrix, and any probable interfering substances likely to be in the same matrix. Further, the null hypotheses of analytical chemistry are cast and tested in terms of electronic signal to noise ratios because modern analytical chemistry is overwhelmingly dependent on electronic instrument responses which are characterized by noise. [Pg.243]

Given a defined set of experimental conditions suitable for observing the effects of the analyte on the properties of the matrix, the probability of observing a signal to noise ratio greater than, or equal to, a predetermined number is not affected by the presence of a specified number of units of the analyte in a specified number of units of the matrix. [Pg.243]

Also known as specific susceptibility. mas sa.sep-ta bil-ad-e mass-to-charge ratio analychem In analysis by mass spectroscopy, the measurement of the sample mass as a ratio to its ionic charge. mas to charj. ra sho ) matrix analy chem The analyte as considered in terms of its being an assemblage of constituents, each with its own properties. ma-triks matrix effects analy chem 1. The enhancement or suppression of minor element spectral lines from metallic oxides during emission spectroscopy by the matrix element (such as graphite) used to hold the sample. 2. The combined effect exerted by the various constituents of the matrix on the measurements of the analysis. ma triks i.feks )... [Pg.231]

In MALDI MS, the analyte is additionally embedded in higher molar excess of matrix molecules, which allows a soft desorption and ionization of the analyte. Typical molar ratios between the matrix and the analyte for measurement of low-molecular weight compounds (<500 Da) are in the range between 10 1 and 100 1, for higher masses (e.g., peptides and proteins) typical ratios are in the range of 1000 1 up to 100,000 1. Despite the capability to be measured by LDI MS, most classical ILs based on pyridinium... [Pg.379]


See other pages where Analyte-to-matrix ratio is mentioned: [Pg.360]    [Pg.4]    [Pg.1196]    [Pg.29]    [Pg.263]    [Pg.360]    [Pg.4]    [Pg.1196]    [Pg.29]    [Pg.263]    [Pg.191]    [Pg.339]    [Pg.35]    [Pg.82]    [Pg.23]    [Pg.337]    [Pg.851]    [Pg.991]    [Pg.201]    [Pg.444]    [Pg.1148]    [Pg.402]    [Pg.71]    [Pg.225]    [Pg.139]    [Pg.458]    [Pg.181]    [Pg.137]    [Pg.413]    [Pg.419]    [Pg.295]    [Pg.187]    [Pg.683]    [Pg.381]    [Pg.69]    [Pg.123]    [Pg.194]    [Pg.389]    [Pg.390]   


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