Analysis bacteria bioassay

Other applications dealt with the development of a luciferin ester substrate to measure the luciferase activity in living cells [141], the detection of toxic compounds such as sodium azide, fluoroacetic acid, and antibiotics [142], the development of a biosensor for the determination of bioavailable mercury [143], the use of eukaryotic luciferases as bacterial markers with different colors of luminescence [144], the determination of complement-mediated killing of bacteria [145], and the development of a bioassay for the determination of HIV type 1 virus and HIV-1 Tat protein activity, valuable also for analysis of HlV-inhibi-tory agents [146],... 

Ecotoxicity assessment of water samples was carried out, in parallel to chemical analysis, using three standardized bioassays based on the micro-crustacean Daphnia magna, the algae Pseudokirchneriella subcapitata, and the bioluminescent bacteria Vibrio fischeri. 

Nowadays, Microtox is the most popular bioassay available that uses bioluminescent bacteria as its active element. Analysts find it a useful tool for assessing pollution in different compartments of the environment, possessing as it does both the advantages of bioindicator techniques and the precision of classic instrumental analysis. Here are some examples of its application ... 

Step 4 Compost analysis for conventional parameters determining any quality change compared with the blind and ecotoxicity test using at least two plant species if negative influences are detected additional bioassays should be performed using more plant species, daphnia, water lens, luminescent bacteria or special local species. 

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