Aminopyrine-N-demethylase

To acquire information on the intrinsic metabolic activity of aquatic organisms, liver of carp (Cyprinus carpio Linnaeus), rainbow trout (Salmo gairdneri) and freshwater snail (Cipango-paludina japonica Martens) was dissected out, homogenized in 0.1M phosphate buffer, pH 7.5, and centrifuged at 105,000 g for 60 min to obtain the microsome-equivalent (described as the microsomal fraction hereafter) fraction. The protein content of microsomal and submicrosomal (supernatant fractions by Lowry s method, microsomal P-450 content ( ), activity of aniline hydroxylase (4) and aminopyrine N-demethylase (5) were determined. 

M. Murray, A.J. Ryan u. J. P. Little. Inhibition of Rat Hepatic Microsomal Aminopyrine N-Demethylases... 

CPH-treatment of rats (1200 mg/kg/d for 2 d) induced the enzymatic activities of other renal cortical drug-metabolizing enzymes such as 7-ethoxy-couma-rine-O-deethylase and cytosolic GSH-S-transferase whereas the enzymatic activities of aniline hydroxylase and aminopyrine-N-demethylase were simultaneously decreased or remained unchanged, respectively (Table 1) [74]. Treatment of male and female rats with cephaloridine (750 mg/kg/d) for two weeks to three... 

Figure 4. Reversal of decreased aniline hydroxylase, aminopyrine N-demethylase and p-nitroanisole O-demethytase activities in vitamin C deficient guinea pigs with ascorbic acid (15).

Ravindranath, V. and Anandatheerthavarada, H.K. (1989) High activity of cytochrome P-450 linked aminopyrine N-demethylase in mouse brain microsomes and associated sex-related differences. Biochem. J. 261 769-773. 

Murray, M., A.J. Ryan, and RJ. Little (1982). Inhibition of rat hepatic microsomal aminopyrine N-demethylase activity by benzimidazole derivatives Quantitative structure-activity relationships. J. Med. Chem. 25, 887-892. 

In rats administered a diet consisting of 5,10, or 30% comfrey (part unspecified) for 21 days, those on the 30% comfrey diet gained less weight than control animals. Rats fed 10 or 30% comfrey or 30% alfalfa Medicago sativd) in the diet exhibited an increase in hepatic aminopyrine N-demethylase activity but had no change in hepatic glutathione S-transferase or epoxide hydrolase activities, as compared to rats fed a control diet (Garrett et al. 1982). 

Aminopyrine N-demethylase Procambus clarkii 225 Jewell and Winston (1989a)... 

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