9- Aminopyrene-1,4,6-trisulfonic acid

The most successful applications of CE-LIF detection have been in the analysis of carbohydrates and nucleic acids. Carbohydrates can be derivatized with agents such as aminopyrene trisulfonic acid, which introduces a fiuorophore and also confers electrophoretic mobility to the analyte. Double-stranded nucleic acids can be detected using intercalating dyes and by covalent attachment of nucleotides carrying fluorophores. The fluorescently labeled primers or chain terminating nucleotides which are widely used in automated slab gel-based DNA sequencers are also used in capillary-based sequencers. In one multicapillary sequencer, a novel sheath flow system is used for LIF excitation of separated bands with imaging of emitted fluorescence on a charge-coupled device (CCD) detector. 

Zhuang et al. [152] derivatized glycan samples with 8-aminopyrene-l,3,6-trisulfonic acid to get a charge for electrophoresis and a fluorescent label for detection. They analyzed glycan derivatives in the blood of cancer patients... 

Complex carbohydrates released from glycoproteins were readily profiled by capillary gel electrophoresis with LIF-based detection of 1-aminopyrene-3,6,8-trisulfonic acid (APTS)-labeled sugar molecules (Figure 10) [125]. High-mannose-type oligosaccharides of ribonuclease B were derivatized by APTS and separated by capillary electrophoresis using polyethylene oxide separation medium [126]. 

Yoshinaka, Y., Ueda, Y., and Suzuki, S. Ion-pair chromatographic separation of glycoprotein-derived oligosaccharides as their 8-aminopyrene-l,3,6-trisulfonic acid derivatives./. Chromatogr. A. 2007, 1143,83-87. 

When separating oligosaccharides in polymer networks with LIF detection, a good tagging reagent is 9-aminopyrene-l,4,6-trisulfonic acid (APTS). This reagent is used in conjunction with the argon-ion laser. 

Aminopyrene-1,3/6-trisulfonic acid trisodium salt (APTS)... 

Other Names 8-Aminopyrene-l,3,6-trisulfonic acid, trisodium salt APTS 1,3,6-Pyrenetrisulfonic acid, 8-amino-, trisodium salt Trisodium 8-aminopyrene-l,3,6-trisulfonate... 

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