Tagging reagents

The use of mass tagging reagents to analyze proteomic data has greatly improved the ability to compare samples for protein expression differences. However, a major limitation of the ICAT procedure (Section 1, this chapter) is that it can only compare two samples simultaneously, usually a test and a control. Even with the ECAT design (Section 2) using multiple lanthanide metals to make a series of different mass tag signatures, it is difficult to extend the... 

Dissolve the isobaric tagging reagents in acetonitrile or ethanol at a concentration of 50 mM (or according to the manufacturer s recommendations). Use a fume hood to handle organic solvents. 

Ross, P.L., Huang, Y.N., Marchese, J.N., Williamson, B., Parker, K., Hattan, S., Khainovski, N., Pillai, S., Dey, S., Daniels, S., Purkayastha, S., Juhasz, P., Martin, S., Bartlet-Jones, M., He, F., Jacobson, A., and Pappin, D.J. (2004) Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents. Mol. Cell. Proteomics 3, 1154-1169. 

Equation 11.32 One-pot hydrostannylation and Stilie reaction with F-21-tagged reagents. 

Filippov DV, van Zoelen DJ, Oldfield SP, van der Marel GA, Overkleeft HS, Drijfhout JW, van Boom JH (2002) Use of Benzyloxycarbonyl (Z)-Based Fluorophilic Tagging Reagents in the Purification of Synthetic Peptides. Tetrahedron Lett 43 7809-7812... 

Scheme 2.29 Rapid purification using tagged reagents for organic synthesis.

A number of polymer assisted procedures have been described that have subsequently become standard practice within the domain of solution-phase hbrary synthesis (complementary molecular reaction and recognition, scavenging, tagged reagent systems and catch and release protocols). A number of these concepts have... 

The role of bifunctional (tagged) reagents in the transformations described above is similar to that of polymer-immobilized reagents. However, they exhibit more reliable reaction kinetics because they react in the solution phase. Moreover, manipulation of these reagents is more easily automated since transfer of solutions is much easier than transfer of solids. 

Polymer-assisted solution-phase synthesis offers strategies for chemical library construction that complement solid-phase methods. Tools now available include chemoselective sequestering resins, bifunctional solution-phase linking reagents, bifunctional chemically tagged reagents, and an... 

There are several immunological techniques in use. Enzyme multiplied immunological technique (EMIT) employs an enzymatic reaction to determine concentration, whereas radioimmunoassay (RIAl uses radioactively tagged reagents such as I lu measure concentration. 

T Toyo oka, YM Liu. Determination of d- and L-amino acid residues in peptides with fluorescent tagging reagents by high-performance liquid chromatography. Chromatographia 40 645-651, 1995. 

Paba, J., Ricart, C.A.O., Fontes, W., Santana, J.M., Teixeira, A.R.L., Marchese, J., Williamson, B., Hunt, T., Karger, B.L. and Sousa, M.V. (2004) Proteomic analysis of Trypanosoma cruzi developmental stages using isotope-coded affinity tag reagents. Journal of Proteome Research 3, 51 7-524. 

Fig. 3. Schematic description of the use of an F-tag reagent for the purification of compounds synthesized on a solid support.

Ross PL, Huang YN, Marchese JN, Williamson B, Parker K, Hattan S, Khainovski N, Pillai S, Dey S, Daniels S, Purayastha S, Juhasz P, Martin S, Bartlet-Jones M, He F, Jacobson A, Pappin DJ (2004) Multiplexed protein quantitation in Saccharmyces cerevisiae using amino-reactive isobaric tagging reagents. Mol Cell Proteomics 3.12 1154-1169... 

Yu, L. R., Conrads, T. P., Uo, T., Issaq, H. J., Morrison, R. S., Veenstra, T. D. (2004). Evaluation of the add-deavable isotope-coded affinity tag reagents application to camptothecin-reated cortical neurons. 

Figure 8.33 Solid-supported scavengers tagging reagents 8.54-8.57 and supported, covalent...

The use of tetrabenzo[a,c,g,i]fluorene (Tbf) derivatives as tagging reagents, such as 8.70 (224), was reported for acids each Tbf-tagged reagent is purified by specific adsorption on charcoal, typically stirring for 20 min in an ice bath, and subsequent washing with lipophilic solvents to recover pure intermediates, typically stirring with toluene aliquots at 40 °C until no more UV adsorbance is detected in the toluene aliquots. 

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