Amino acid sequences numbering system

Recombinant DNA techniques have provided tools for the rapid determination of DNA sequences and, by inference, the amino acid sequences of proteins from structural genes. The number of such sequences is now increasing almost exponentially, but by themselves these sequences tell little more about the biology of the system than a New York City telephone directory tells about the function and marvels of that city. 

The successful expression of recombinant plant peroxidases such as HRP C has been a major focus of research in a number of laboratories. Three synthetic HRP C genes based on the amino acid sequence determined by Welinder (36, 47) were synthesized independently in order to initiate this work (63-65). A number of different expression systems have been evaluated (64, 66-73), a summary of which is presented in Table I. Refolding of recombinant HRP C isolated from inclusion... 

With respect to a solvent, the overall solvation capability for solutes. 2. A property of bodies or systems that have a distinct direction i.e., that have different or opposing physical properties or characteristics at different points. For example, an amino acid sequence in a polypeptide has polarity in that there is an amino end and a carboxyl end of the sequence. Similarly, microtubules and actin filaments have plus (+)-ends and minus (-)-ends that establish directionality for cellular and intracellular locomotion. 3. The state in which there is either a positive or negative aspect relative to the two poles of a magnet or to electrification. 4. Attraction toward an object or attraction in a specific direction. 5. In mathematics, the positive or negative sign of numbers. 

Amino acid sequences of Type 1 sea anemone polypeptides. The residue numbering system is based on the sequences of AP-A and AP-B. Literature references are from Norton [24], except in the case of recently published sequences for Be III [25], Bg II and Bg III... 

In order to be able to predict the retention behavior of peptides of different composition, of peptides of the same composition but different sequence (positional isomers), and of diastereoisomeric peptides, a knowledge of the incremental contribution of each amino acid to the overall contact area term is required not only for each well-defined stationary phase but also for each mobile-phase condition. Group retention coefficient summation approaches based on the assumption that selectivity differences can be ascribed predominantly to amino acid sequence differences, have been developed by Meek (46a, 52b) and Su et al. (45a). These treatments have subsequently been applied to a number of different elution systems (52c-52e). A comparative analysis of the different amino acid group contribution coefficients derived for phosphate, perchlorate, pyridine/acetate, trifluoroacetate, and bicarbonate buffer systems has been reported (52f). 

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