Alternatively spliced forms

The smooth endoplasmic reticulum calcium pumps (SERCA) found in brain were first identified in sarcoplasmic reticulum. The three isoforms of SERCA are products of separate genes SERCA-1 is expressed in fast-twitch skeletal muscle SERCA-2a in cardiac/slow-twitch muscle SERCA-2b, an alternatively spliced form, is expressed in smooth muscle and non-muscle tissues SERCA-3 is... 

The discovery of new alternative splice forms of genes associated with disease has the exciting potential to lead to new rapid PCR-based diagnostic markers. The ability to extract such alternative splice forms together... 

Steinman, H. a., et ah. An Alternative Splice Form of Mdm2 Induces p53-independent Cell Growth and Tumorigenesis. J Biol Chem, 2004, 279(6), 4877-86. 

RT-PCR, Southern biot, sequencing and gene-specific PCR characterize alternatively spliced forms of CYP2D mRNA in human breast tissue (Huang et al., 1997). 

Tremendous strides have been taken in understanding the metabolism and biosynthesis of fibrinogen, but there is still much to be done. Almost nothing is known of the catabolism of fibrinogen. The functions of the alternately spliced forms and the many posttranslational modifications are only partly known. Many dysfibrinogenemias have been discovered, but most have only been relatively superficially characterized. 

Segade, F., Trask, B. C., Broekelmann, T. J., Pierce, R. A., and Mecham, R. P. (2002). Identification of a matrix binding domain in the microfibril-associated glycoproteins 1 and 2 (MAGP1 and 2) and intracellular localization of alternative splice forms./. Biol. Chem. 277, 11050-11057. 

Eliasson MJ, Blackshaw S, Schell MJ, Snyder SH (1997) Neuronal nitric oxide synthase alternatively spliced forms prominent functional localizations in the brain. Proc Natl Acad Sci USA 94 3396—401... 

Able to detect modifications and alternative splice forms in addition to abundance... 

Wu X, Freeze HH. GLUT14, a duplicon of GLUT3, is specifically expressed in testis as alternative splice forms. Genomics 2002 80 553-557. 

Neve, K.A. Neve, R.L. Fidel, S. Janowski, A. Higgins, G.A. (1991) Increased abundance of alternative spliced forms of D2 dopamine receptor mRNA after denervation. Proc. Natl. Acad. Sci. USA 88, 2802-2806. 

Comprehensive transcriptome profiling studies using RNA-Seq (124) have found evidence for many novel exons, and the number of identified alternative splicing forms is much larger than previously expected (137). As alternatively spliced gene structures can be very different from the initial predicted gene structure, current ab initio and sequence similarity methods are not adequate to validate such predictions. Instead, mRNA sequences from RNA-Seq are translated to proteins and used as databases to identify the protein products of these novel exons, even though the final number of sequences that actually become functional proteins in vivo remains unknown (138,139). 

---