Allergy basophil activation

If patients have experienced anaphylaxis, the identification of any possible elicitor is important to help avoid further episodes. With skin tests and specific IgE antibodies combined with history, a relevant allergy may be detected. Cellular tests monitoring basophil histamine release or basophil activation may be helpful in some patients who resist diagnosis by standard means [26,31]. 

Sanz ML, Gamboa PM, Antepara I, Uasuf C, Vila L, Garda-Aviles C, Chazot M, De Week L Flow cytometric basophil activation test by detection of CD63 expression in patients with immediate-type reactions to (J-lactam antibiotics. Clin Exp Allergy 2002 32 277-286. 

Gamboa P. Sanz ML. Caballero MR. Urrutia I. 43 Antepara I. Esparza R. De Week L The flow-cytometric determination of basophil activation induced by aspirin and other non-steroidal and anti-inflam- 44 matory drugs (NSAIDs) is useful for in vitro diagnosis of the NSAID hypersensitivity syndrome. Clin dS Exp Allergy 2004 34 1448-1457. 

Gamboa PM. Sanz ML. Caballero MR. Antepara I. Urrutia I. Gonzalez G. Dieguez I. De Week AL Use of CD63 expression as a marker of in vitro basophil activation and leukotriene determination in methi- 46 mazole allergic patients. Allergy 2003 58 312-317. 

Garda BE, Barber D, Salcedo G, Rihs HP, Raulf-Heimsoth M Basophil activation test and specific IgE measurements using a panel of recombinant natural rubber latex allergen to determine the latex allergen sensitization profile in children. Pediatr 52 Allergy Immunol 2006 17 148-156. 

Eberlein-Konig B. Rakoski J, Behrendt H, Ring J Use of CD63 expression as marker of in vitro basophil activation in identifying the culprit in insect venom allergy. J Investig Allergol Chn Immunol 2004 14 10-16. 

Positive basophil activation tests were reported in patients with immediate RCM hypersensitivity reactions, which may be regarded as another indirect indication for an IgE-mediated allergy [35]. 

R. Kwiecien, B. Maderegger, and K. Hoffmann-Sommergruber. 2005. In vitro analysis of birch-pollen-associated food allergy by use of recombinant allergens in the basophil activation test. Int Arch Allergy Immunol 136 (3) 230-238. 

Monneret G, Benoit Y, Gutowski MC, Bienvenu J. Detection of basophil activation by flow cytometry in patients with allergy to muscle-relaxant drugs. Anesthesiology 2000 92(l) 275-7. 

Manso L, Heili S, Femandez-Nieto M, Sastre B, Sastre J. Basophil activation in two cases of hydrochlorothiazide-induced noncardiogenic pulmonary edema. Allergy 2010 65(1) 135-6. 

In contrast to the above results, the basophil activation test was positive to the implicated quinolone in all five subjects with immediate reactions to a quinolone who were tested [19 ]. However, this assay quantified CD203C, which may be a more sensitive marker than CD63 for detecting IgE-mediated allergy. 

Ben Said B, Berard F, Bienvenu J, Nicolas JF, Rozieres A. Usefulness of basophil activation tests for the diagnosis of IgE-mediated allergy to quinolones. Allergy 2010 65(4) 535-6. 

Valent P. Basophil activation antigens molecular mechanisms and clinical implications. Open Allergy J. 2010 3 52-9. 

When used as a diagnostic aid for allergy to NMBDs, the basophil activation test (BAT) has been found to be specific but disappointingly lacking in sensitivity. Results for specificity have generally been above 90 %, and although a figure... 

Dewachter P, Nicaise-Roland P, Kalaboka S, Lefevre J, Chollet-Martin S. Anaphylaxis to amidatrizoate proved by skin testing and flow cytometry-based basophil activation test. Allergy 2009 64 496-502. 

Lie WJ, Homburg CH, Kuijpers TW, Knol EF, Mul FR Roos D, Tool AT Regulation and kinetics of platelet-activating factor and leukotriene C4 synthesis by activated human basophils. Clin Exp Allergy 2003 33 1125-1134. 

Forster E, Dudler T, Gmachl M, Aberer W, Utbanek R, Suter M Natural and recombinant enzymatically active or inactive bee venom phospholipase A2 has the same potency to release histamine from basophils in patients with hymenoptera allergy. J Allergy Clin Immunol 1995 95 1229-1235. 

The PMN cells include neutrophils, eosinophils, and basophils. Neutrophils make up 60-75% of circulating WBCs and provide the first line of defense against microbes that penetrate the normal barriers of skin. They are extremely efficient phagocytes and are a source of inflammatory cytokines and lipid mediators such as PGs, LTs, and platelet-activating factor. Eosinophils are involved in allergy and provide protection against parasites. Basophils contain vasoactive amines such as histamine, serotonin, and heparin, as well as precursors for PGs and LTs. Release of these pharmacological materials by the basophils is responsible for the anaphylactic reaction. These factors also serve as chemoattractants for neutrophils and eosinophils to sites of inflammation. 

Marone G, Rossi FW, Bova M, Detoraki A, Liccardo B, Petraroli A Superallergens a novel mechanism of IgE-mediated activation of human basophils and mast cells. Clin Exp Allergy Rev 2004 4(suppl 2) 64-75. 

Marone G, Poto S, Petracca R, Triggjani M, de Lutio di Castelguidone E, Condorelli M Activation of human basophils by staphylococcal protein A. 1. The role of cyclic AMP, arachidonic acid metabolites, microtubules and microfilaments. Clin Exp Allergy 1982 50 661-668. 

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