Alcohols fluorescent labelling

Nucleophilic ring opening of salicyl alcohol acetals was effected by borane-trimethylamine upon Lewis acid activation with AICI3. This reaction was particularly useful for the construction of fluorescently labeled compounds (Equation 29) <2006JOC3444>. 

Confocal laser scanning microscopy can be used in conjunction with microwave heating for examining the three-dimensional structure and cellular interrelationships in sections of paraffin-embedded tissues (Boon and Kok, 1994). Tissues are fixed with Kryofix, a coagulant fixative containing 50% ethyl alcohol and polyethylene glycol (PEG molecular weight 300) for 90 sec in a microwave oven. The use of thick paraffin sections (15 (xm) and fluorescently labeled antibodies is preferred. 

Table 10.6 compares the average size (DPn) and size distributions of six laboratory-purified amyloses and one commercial sample of potato amylose, which were determined by classic colorimetric and fluorescent-labeling techniques using 2-ami-nopyridine. The data by the two techniques are consistent and show that wheat and other cereal amyloses are smaller in size than those from root and tuber starches. The molar distribution technique indicated that wheat amylose contained two molecular species, compared with one for rice and com amyloses.209,210 Moreover, the molar size distributions for the cereal amyloses are much narrower than those of the tuber amyloses, and the cereal amyloses contain a preponderance of molecules of DPn < 1000 whereas the tuber amyloses contain 78-95% of molecules with DPn > 1000, and even 3-5% above DPn 10000. None of the amylose samples in Table 10.6 showed molecules with less than DPn 200, possibly because they had been purified as alcohol-inclusion complexes.209... 

Alcohols suitable for fluorescence labelling of activated carboxylic acids... 

Dimethylamino-l-naphthoylnitrile [500], 1- and 9-anthroylnitrile [501] and 2-methyl-l,l -binaphthalene-2 -carbonylnitrile [502] have been suggested as fluorescent labels for primary and secondary alcohols. 2-Methyl-l,l-binaphthalene-2 -carbonylnitrile is a chiral reagents, and is therefore suited for the separation of enantiomers. Quantitative reaction is achieved by heating in the presence of triethylamine, whereby the diastereo-meric esters are formed. 

Dehydrative Condensation of Carboxylic Acids with Amines and Alcohols. For reversible fluorescent labeling of amino groups, ethoxy(trimethylsilyl)acetylene was used as a dehydrating reagent to couple dansylaminomethylmaleic acid (DAM) with benzylamine through a maleic anhydride intermediate (eq 6). ... 

Tsuruta, Y. Date, Y. Kohashi, K. (2-Phthalimidyl)benzoylazides as fluorescence labeling reagents for alcohols in high-performance liquid chromatography, A a/.Sci., 1991, 7, 411-414. 

Fluorescence labelling reagent for glc anal, of steroidal alcohols. Dark yellow solid. 

Fluorescent labelling probe for alcohols and carboxylic acids. Yellow needles. Mp 160° dec. 

Fluorescent labelling reagent for alcohols. Mp 137-138°. Takadate, A. et al, Yakugaku Zasshi, 1983, 103, 962 synth, use)... 

Fluorescent label for alcohols and amines. Faint greenish-yellow plates (EtOH aq.). Mp 195°. 

Fluorescent labelling reagent for alcohols. Pale yellow needles. Mp 170-171°. 

Fluorescence labelling reagent for alcohols in hplc. Needles (Me2CO aq.). Mp 123-126°. 

Flexographic Inks. Fluorescent toners such as the Radiant GF, Lawter HVT, and Day-Glo HM and HMS Series toners are used in flexographic ink formulations. These products are soluble in blends of alcohol (80%) and ester solvents (20%) and are compatible with modifying materials such as nitroceUulose resins and acryHc solution polymers. Flexographic inks of this type are used most commonly to print products such as ceUophane and polyethylene film for packaging, and also to print paper products such as gift wrap and price labels. 

Takadate, A., Irikura, M., Suehiro, T., Fujino, H., and Goya, S. (1985) New labeling reagents for alcohols in fluorescence high-performance liquid chromatography. Chem. Pharm. Bull. 33, 1164-1169. 

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