Agilent ChemStation

Factor MF (Agilent ChemStation), or Similarity Index SI (Shimadzu Class-VP, Chromatography Data System or Shimadzu/LCsolution). 

Abstract A relatively small number of mammalian pheromones has been identified, in contrast to a plethora of known insect pheromones, but two remarkable Asian elephant/insect pheromonal linkages have been elucidated, namely, (Z)-7-dodecen-1-yl acetate and frontalin. In addition, behavioral bioassays have demonstrated the presence of a chemical signal in the urine of female African elephants around the time of ovulation. Our search for possible ovulatory pheromones in the headspace over female African elephant urine has revealed for the first time the presence of a number of known insect pheromones. This search has been facilitated by the use of a powerful new analytical technique, automated solid phase dynamic extraction (SPDE)/GC-MS, as well as by novel macros for enhanced and rapid comparison of multiple mass spectral data files from Agilent ChemStation . This chapter will focus on our methodologies and results, as well as on a comparison of SPDE and the more established techniques of solid phase microextraction (SPME) and stir bar sorptive extraction (SBSE). 

New FindPeak Macros for Organization and Comparison of Mass Spectral Data Output from Agilent ChemStation Software... 

TIC) for each urine sample. For comparison of TICs, Agilent ChemStation software allows one easily to stack (overlay) several chromatograms in the same computer window (Fig. 2.2). This feature helps to identify compounds with common retention times among several samples, but does not contain any mass spectral data for verification of a common identity for these peaks and is most useful for comparison of only a few TICs or a few compounds of interest. In these cases, mass spectral comparisons can be made quickly by visual inspection of the relevant spectra. 

In brief, the macros carry out the following steps (1) Agilent ChemStation ... 

Agilent ChemStation software, 29-31, 33 Alkylquinaxolines, in Phodopus roborovskii, 19 Allomones, 13 2-Aminoacetophenon, 155 2-Amino-7-hydroxy-5,5-dimethyl-4-thiaheptanoic acid, see Felinine Amniotic fluid, odour of, 194 Amphetamine, 266... 

Figure 4.18. Graphical user interface of Agilent ChemStation showing the status of various HPLC modules in the system and a real-time chromatographic plot.

Agilent ChemStation is a well-proven and a popular software product. Over the years, the software has achieved a high degree of distribution, particularly at universities and public institutions. The software design of ChemStation has become out-of-date over the years. That s why Agilent has started a redesign, calling the software now OpenLAB. 

Agilent 1100 LC and LC/MS systems with Agilent ChemStation HPLC software (Agilent Technologies GmbH, Waldbronn, Germany). 

Agilent GC-MS ChemStation System equipped with capillary gas chromatograph HP 5972,5973, or 6890 series Agilent 6890 series autosampler. The GC-MS system is operated in the positive-ion, electron-impact ionization mode. Chromatographic parameters of the acquisition method are listed in Table 3.1.7. TIC is recorded over the mass range m/z 50-550, and the total run time is 33 min. 

Agilent 1100 liquid chromatographic system equipped with thermostated autosampler, binary pump, photo diode array detector and Chemstation software (Agilent, USA). 

Figure 4.11 Quantitative analysis software for chromatography. Since the signal from the detector situated at the outlet of the column is analogical, an analogue-digital converter (ADC) is necessary. The stored chromatogram, digitized, serves as a basis for its exploitation hy the software. Different zones of the screen can display the calibration curve, methodology, etc. (software Chemstation from Agilent Technologies).

HPLC/DAD/ESI-MS/MS analyses LC/ESI-MS/MS experiments were performed on Agilent 1100 LC/MSD Trap-SL mass spectrometer (Palo Alto, CA) equipped with an electrospray ionization (ESI) interface, 1100 HPLC, a DAD detector and Chemstation software (Rev.A.09.01). Anthocyanins were monitored at 520 nm. The colunrn used was a 150 cm x 2.0 mm i.d., 4 pm... 

Data collection was performed with Agilents GC ChemStation software revision A.06.03. Although ChemStation software is in-use at DuPont, the MARS units typically used more traditional Laboratory Information Management Systems (LIMS) for GC data management. 

A Hewlett Packard Vectra Pentium IV personal computer operating under Microsoft Windows NT 4.0 and Agilent Technologies A.07.01 ChemStation software was used for data acquisition and instrument control. Two dimension plots were obtained with Fortner Software Transform Version 3.4 after the signal files were exported in CSV file format by the ChemStation software. 

The gradient run was set at 100% A from 0 to 8 min, then to 100% B until 14.5 min, where it held until minute 17. The system reequilibration was performed by changing to 100% A from minute 17 to 18 and remaining with 100% A until minute 20. All operations and data analysis were processed by the Chemstation software v.8.03 (Agilent Technologies, Inc., Santa Clara, CA). 

The process of picking single beads from encoded library pools and assessing both the codes and the ligand on each bead were described in literature. This process has been automated through the use of a robotic beadpicker. Experiments were conducted on an Agilent LC/MSD instrument controlled by ChemStation Rev. A08.04. 

In 2010, Agilent started launching rtgi/ewt OpenLAB CDS. Agilent talks about an evolution of its software instead of calling it a new CDS. Two versions are currently available on the market the ChemStation and the EZchrom edition. Both of them use the same control panel module and the same report generator. All other software parts are adjusted to the customs of ChemStation and EZchrom, respectively. 

Agilent has obtained the EZchrom software with the acquisition of Scientic Software. Over the years, EZchrom has become the core part of many other CDS. Just as ChemStation, the EZchrom software has been revised and is called OpenLAB CDS EZchrom Edition. 

Fig. 1. Forced stress test. Results of automatic optimization of the separation of an active pharmaceutical ingredient (API) impurities and degradation products are present at concentration levels of 0.01-0.7% relative to the API. Software ChromSword 3.1, ChemStation 10.2. Agilent 1100 HPLC system. Column Inertsil ODS-3, 5 pm, 15 cm x 4.6 mm.

Chromatography Software Optimization Utility Empower (Waters) DryLab 2000 Plus (LC Resources) Chemstation (Agilent), Empower (Waters) or EZChrom (VWR) Expert system (Galushko, VWR) Chemstation (Agilent) Development (Prof Otto, Univ. Freiberg AnaConDa, Schering)... 

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