Affinity chromatography reaction with cyanogen bromide

Method A. A high molecular weight polysaccharide was activated by reaction with cyanogen bromide at basic pH, in a similar fashion Sepharose is activated for affinity chromatography. The reaction takes place at all possible hydroxy groups to produce 0-CN randomly... 

Derivatives of cellulose continue to be used as supports for new affinity chromatography media and for immobilization of enzymes. Trypsin has been immobilized on 4-aminobenzyl-cellulose. A penicillinase from Bacillus cereus has been immobilized by glutaraldehyde-mediated reaction with aminoethyl-cellulose. Tannin has been activated with cyanogen bromide and coupled with... 

Carbonates are also formed when carbohydrates are reacted with cyanogen bromide, followed by reaction with a second molecule containing a reactive amine or alcohol group. These reactions are used to couple various ligands such as enzymes, antibodies, affinity ligands, and so forth to an insoluble carbohydrate carrier for use in affinity chromatography or for the immobilization of enzymes (see section 7.8 in Chapter 7). 

Polysaccharides and cross-linked polysaccharides can be activated by reaction with alkaline cyanogen bromide (reaction 7.19). Polysaccharides such as cross-linked dextran, agaran, and cellulose have been activated by this reaction [37,38]. The activated polysaccharides can then react with amino groups of various ligands that can be used in affinity chromatography [39], or they can react with amino groups on the surface of enzymes to give immobilized enzymes [40] (reaction 7.20). 

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